PXD004024

PXD004024 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	MITOCHONDRIAL Akt REGULATION OF HYPOXIC METABOLIC REPROGRAMMING
Description	Hypoxia is a driver of aggressive tumor behavior, but the mechanisms are not completely understood. In a global phosphoproteomics screen, we now demonstrate that hypoxia induces the recruitment of Akt2 to tumor mitochondria, and Akt phosphorylation of pyruvate dehydrogenase kinase (PDK1) on Thr346. In turn, Akt-phosphorylated PDK1 shuts off oxidative phosphorylation via phosphorylation of the pyruvate dehydrogenase complex, and reprograms tumor metabolism towards glycolysis. Akt-phosphorylation of PDK1 is required to prevent autophagy, maintain cell viability and support tumor cell proliferation in hypoxia, in vivo. Therefore, mitochondrial Akt is a pathophysiologic switch for tumor adaptation in hypoxia.
HostingRepository	MassIVE
AnnounceDate	2016-04-21
AnnouncementXML	Submission_2016-04-21_07:25:55.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hsin-Yao Tang
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Phospho
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2016-04-20 18:46:55	ID requested
⏵ 1	2016-04-21 07:25:56	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: LC-MS/MS, SILAC, Phosphoproteome, Akt, Mitochondria

submitter keyword: LC-MS/MS, SILAC, Phosphoproteome, Akt, Mitochondria

Contact List

Dario C. Altieri
lab head
Hsin-Yao Tang
contact affiliation	The Wistar Institute
contact email	tangh@wistar.org
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000079671