PXD004019 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | AP-MS analysis of m7GTP-binding proteins in Murine norovirus infected BV-2 cells |
Description | SILAC-labelling analysis of murine BV-2 cells infected at high multiplicity of infection (10 TCID50/cell) and harvested at 4h or 9h post-infection. Lysates were then suject to m7GTP-sepharose affinity purification to enrich for translation initiation factors. As norovirus translation uses a VPg protein covalently linked to the 5' of its RNAs for initiation factor recruitment it was hypothesised that norovirus infection could modify initiation factor complexes. A paired dataset investigates the effects of MNV infection on total protein levels in infected cells (PXD004015). |
HostingRepository | PRIDE |
AnnounceDate | 2016-07-08 |
AnnouncementXML | Submission_2016-07-08_00:10:33.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Edward Emmott |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | isotope labeled residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-04-20 03:01:07 | ID requested | |
⏵ 1 | 2016-07-08 00:10:34 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biomedical |
submitter keyword: SILAC, Norovirus, MNV, BV-2, eIF, initiation, translation, m7GTP |
Contact List
Ian Goodfellow |
contact affiliation | Division of Virology, Department of Pathology, University of Cambridge, UK |
contact email | ig299@cam.ac.uk |
lab head | |
Edward Emmott |
contact affiliation | University of Cambridge |
contact email | ee273@cam.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004019
- Label: PRIDE project
- Name: AP-MS analysis of m7GTP-binding proteins in Murine norovirus infected BV-2 cells