PXD004016

PXD004016 is an original dataset announced via ProteomeXchange.

Title	Proteomics offers insight to the mechanism behind Pisum sativum L. response to Pea Seed-borne Mosaic Virus (PSbMV)
Description	Pea seed-borne mosaic virus (PSbMV) significantly reduces yields in a broad spectra of legumes. The eukaryotic translation initiation factor has been shown to confer resistance to this pathogen, thus implying that translation and proteome dynamics play a role in resistance. This study presents the results of a proteome-wide analysis of Pisum sativum L. response to PSbMV infection. LC-MS profiling of two contrasting pea cultivars, resistant (B99) and susceptible (Raman) to PSbMV infection, detected >2300 proteins, 116 of which responded to PSbMV ten and/or twenty days post-inoculation. These differentially abundant proteins are involved in number of processes that have previously been reported in the plant-pathogen response, including protein and amino acid metabolism, stress signaling, redox homeostasis, carbohydrate metabolism, and lipid metabolism. We complemented our proteome-wide analysis work with targeted analyses of free amino acids and selected small molecules, fatty acid profiling, and enzyme activity assays. Data from these additional experiments support our findings and validate the biological relevance of the observed proteome changes. We found surprising similarities in the resistant and susceptible cultivars, which implies that a seemingly unaffected plant, with no detectable levels of PSbMV, actively suppresses viral replication.
HostingRepository	PRIDE
AnnounceDate	2016-05-31
AnnouncementXML	Submission_2016-05-31_05:05:47.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD004016
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Martin Cerny
SpeciesList	scientific name: Pisum sativum (Garden pea); NCBI TaxID: 3888;
ModificationList	Oxidation; Acetyl; Deamidated
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2016-04-19 07:32:00	ID requested
⏵ 1	2016-05-31 05:05:48	announced

Cerna H, Č, ern, ý M, Hab, á, nov, á H, Š, af, á, ř, ov, á D, Abushamsiya K, Navr, á, til M, Brzobohat, ý B, Proteomics offers insight to the mechanism behind Pisum sativum L. response to pea seed-borne mosaic virus (PSbMV). J Proteomics, 153():78-88(2017) [pubmed]

curator keyword: Biological

submitter keyword: Proteome

Pea seed-borne mosaic virus PSbMV

potyvirus

resistance

LC-MS

Martin Cerny
contact affiliation	Mendel University in Brno, CEITEC MENDELU
contact email	martincerny83@gmail.com
lab head
Martin Cerny
contact affiliation	Mendel University in Brno
contact email	martincerny83@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004016
     1. Label: PRIDE project
     2. Name: Proteomics offers insight to the mechanism behind Pisum sativum L. response to Pea Seed-borne Mosaic Virus (PSbMV)