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PXD004011

PXD004011 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLC-MS/MS data of Sulfurospirillum multivorans
DescriptionOrganohalide respiration is an environmentally important but poorly characterized type of anaerobic respiration. We compared the global proteome of the versatile organohalide-respiring Epsilonproteobacterium Sulfurospirillum multivorans grown with different electron acceptors (fumarate, nitrate, or tetrachloroethene [PCE]).The most significant differences in protein abundance were found for gene products of the organohalide respiration region. This genomic region encodes the corrinoid and FeS cluster containing PCE reductive dehalogenase PceA and other proteins putatively involved in PCE metabolism such as those involved in corrinoid biosynthesis. The latter gene products as well as PceA and a putative quinol dehydrogenase were almost exclusively detected in cells grown with PCE. This finding suggests an electron flow from the electron donor such as formate or pyruvate via the quinone pool and a quinol dehydrogenase to PceA and the terminal electron acceptor PCE. Two putative accessory proteins, an IscU-like protein and a peroxidase-like protein, were detected with PCE only and might be involved in PceA maturation. The proteome of cells grown with pyruvate instead of formate as electron donor indicates a route of electrons from reduced ferredoxin via an Epsilonproteobacterial complex I and the quinone pool to PCE.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:29:00.972.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNico Jehmlich
SpeciesList scientific name: Sulfurospirillum multivorans DSM 12446; NCBI TaxID: 1150621;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-04-19 03:19:53ID requested
12016-07-15 00:38:25announced
22024-10-22 04:29:01announced2024-10-22: Updated project metadata.
Publication List
Goris T, Schiffmann CL, Gadkari J, Schubert T, Seifert J, Jehmlich N, von Bergen M, Diekert G, Proteomics of the organohalide-respiring Epsilonproteobacterium Sulfurospirillum multivorans adapted to tetrachloroethene and other energy substrates. Sci Rep, 5():13794(2015) [pubmed]
10.1038/srep13794;
Keyword List
curator keyword: Biological
submitter keyword: Organohalide respiration,Epsilonproteobacterium, tetrachloroethene, Proteomics
Contact List
Martin von Bergen
contact affiliationHelmholtz Centre for Environmental Research – UFZ Department of Molecular Systems Biology Permoserstr. 15 04318 Leipzig, Germany
contact emailmartin.vonbergen@ufz.de
lab head
Nico Jehmlich
contact affiliationHelmholtz-Centre for Environmental Research - UFZ
contact emailnico.jehmlich@ufz.de
dataset submitter
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Dataset FTP location
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