PXD004003

PXD004003 is an original dataset announced via ProteomeXchange.

Title	Determination of the interactome of Non-Structural Protein12 from Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus with Host Cellular Proteins UsingHigh Throughput Proteomics and Identification of Hsp70 as a Critical Cellular Factor for Virus Biology
Description	Porcine reproductive and respiratory syndrome virus (PRRSV) has caused tremendous economic losses and continues to be a serious problem to the swine industry worldwide. Although extensive research has been focused on PRRSV, the structure and function of some viral proteins like nonstructural protein12 (NSP12), which may play important roles in viral replication and production, still remain unknown. In order to better understand the function of NSP12, we investigated the interaction of NSP12 with cellular proteins using quantitative proteomics coupled with an immune-precipitation strategy based on the over expression of an NSP12-EGFP fusion protein in 293T cells. Data analysis identified 112 cellular proteins interacted with NSP12-EGFP with high probability. The majority of those proteinsare nucleic acid binding proteins or chaperones, which are involved in RNA post-transcriptional modification, protein synthesis and cellular assembly and organization.Among them, cellular chaperon Hsp70 was verified to interact with PRRSV NSP12 protein, and inhibition of HSP70 significantly reduced the viral mRNA synthesis and virus replication. Our data suggested that NSP12 could recruit cellular proteins such as HSP70 to maintain its own stability and benefit for the virus replication.
HostingRepository	PRIDE
AnnounceDate	2016-06-27
AnnouncementXML	Submission_2016-06-27_04:22:22.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stuart Armstrong
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-04-19 01:18:09	ID requested
⏵ 1	2016-06-27 04:22:23	announced

Dong S, Liu L, Wu W, Armstrong SD, Xia D, Nan H, Hiscox JA, Chen H, Determination of the interactome of non-structural protein12 from highly pathogenic porcine reproductive and respiratory syndrome virus with host cellular proteins using high throughput proteomics and identification of HSP70 as a cellular factor for virus replication. J Proteomics, 146():58-69(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: HP-PRRSV, NSP12, HSP70, label free proteomics, interactome, inhibitor study

Julian Hiscox
contact affiliation	Department of Infection Biology, Institute of Infection and Global Health, University of Liverpool,
contact email	julianh@liverpool.ac.uk
lab head
Stuart Armstrong
contact affiliation	Infection Biology
contact email	sarmstro@liv.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/06/PXD004003

PRIDE project URI

• PRIDE
  1. PXD004003
     1. Label: PRIDE project
     2. Name: Determination of the interactome of Non-Structural Protein12 from Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus with Host Cellular Proteins UsingHigh Throughput Proteomics and Identification of Hsp70 as a Critical Cellular Factor for Virus Biology