PXD003972

PXD003972 is an original dataset announced via ProteomeXchange.

Title	Dynamic of the Grb2 interactome in primary cells by DIA-MS
Description	We applied AP-SWATH to quantify Grb2 interaction dynamics in primary T cells with a high level of reproducibility and accuracy. We concluded that AP-SWATH is a robust approach to quantify signaling dynamics in primary cells and could be further expanded to additional hub proteins using a range of cell and tissues types.
HostingRepository	PRIDE
AnnounceDate	2017-03-09
AnnouncementXML	Submission_2017-04-03_02:13:36.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Etienne Caron
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2016-04-14 07:16:33	ID requested
1	2017-03-09 01:27:05	announced
⏵ 2	2017-04-03 02:13:37	announced	Updated publication reference for PubMed record(s): 28355572.

Caron E, Roncagalli R, Hase T, Wolski WE, Choi M, Menoita MG, Durand S, Garc, í, a-Blesa A, Fierro-Monti I, Sajic T, Heusel M, Weiss T, Malissen M, Schlapbach R, Collins BC, Ghosh S, Kitano H, Aebersold R, Malissen B, Gstaiger M, Precise Temporal Profiling of Signaling Complexes in Primary Cells Using SWATH Mass Spectrometry. Cell Rep, 18(13):3219-3226(2017) [pubmed]

curator keyword: Biological

submitter keyword: Grb2, interactome, primary T cells, SWATH, DIA-MS

Matthias Gstaiger
contact affiliation	Department of Biology, Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland
contact email	matthias.gstaiger@imsb.biol.ethz.ch
lab head
Etienne Caron
contact affiliation	ETH Zurich
contact email	caron@imsb.biol.ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/03/PXD003972

PRIDE project URI

• PRIDE
  1. PXD003972
     1. Label: PRIDE project
     2. Name: Dynamic of the Grb2 interactome in primary cells by DIA-MS