PXD003946

PXD003946 is an original dataset announced via ProteomeXchange.

Title	Biochemical Foundations of Health and Energy Conservation in Hibernating Free-Ranging Subadult Brown Bear Ursus arctos
Description	Brown bears (Ursus arctos) hibernate for 5-7 months without eating, drinking, urinating and defecating at a metabolic rate of only 25% of the summer activity rate. Nonetheless, they emerge healthy and alert in spring. We analyzed the physiological adaptations for hibernation by comparing the proteome, metabolome, and hematologic features of blood from hibernating and active free-ranging sub-adult brown bears with a focus on conservation of health and energy. We found that the concentrations of most plasma proteins and white blood cell types decreased during hibernation. Strikingly, antimicrobial defense proteins increased in concentration. Central functions in hibernation involving the coagulation response and protease inhibition, as well as lipid transport and metabolism, were upheld by increased levels of very few key or broad-specificity proteins. A dramatic 45-fold increase in sex-hormone-binding-globulin SHBG levels during hibernation draws, for the first time, attention to its significant but unknown role in maintaining hibernation physiology. We propose that energy for the costly protein synthesis is reduced by three mechanisms, (i) dehydration, which increases protein concentration without de novo synthesis; (ii) reduced protein degradation rates due to a 6°C reduction in body temperature, decreased protease activity, and increased plasma viscosity; and (iii) a marked redistribution of energy resources only increasing de novo synthesis of few key proteins. This comprehensive global data identified novel biochemical strategies for bear adaptations to the extreme condition of hibernation, and have implications for our understanding of physiology in general.
HostingRepository	PRIDE
AnnounceDate	2016-09-30
AnnouncementXML	Submission_2016-09-30_02:03:43.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Malene Brohus
SpeciesList	scientific name: Ursus arctos arctos (European brown bear); NCBI TaxID: 563924;
ModificationList	iodoacetic acid derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-04-12 08:06:52	ID requested
1	2016-09-27 14:25:31	announced
⏵ 2	2016-09-30 02:03:44	announced	Updated project metadata.

Welinder KG, Hansen R, Overgaard MT, Brohus M, S, ø, nderk, æ, r M, von Bergen M, Rolle-Kampczyk U, Otto W, Lindahl TL, Arinell K, Evans AL, Swenson JE, Revsbech IG, Fr, ø, bert O, Biochemical Foundations of Health and Energy Conservation in Hibernating Free-ranging Subadult Brown Bear Ursus arctos. J Biol Chem, 291(43):22509-22523(2016) [pubmed]

curator keyword: Biological

submitter keyword: Brown Bear, Hibernation, LC-MS/MS, Proteomics, Ursus arctos

Karen G Welinder
contact affiliation	Professor of Biotechnology Aalborg University, Department of Chemistry and Bioscience Fr Bajers Vej 7H 9220 Aalborg East, Denmark
contact email	kgw@bio.aau.dk
lab head
Malene Brohus
contact affiliation	Aalborg University
contact email	mbrohus@bio.aau.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003946
     1. Label: PRIDE project
     2. Name: Biochemical Foundations of Health and Energy Conservation in Hibernating Free-Ranging Subadult Brown Bear Ursus arctos