PXD003940

PXD003940 is an original dataset announced via ProteomeXchange.

Title	Phosphatase POPX2 exhibits dual regulatory functions in cancer metastasis
Description	Cancer metastasis is a complex mechanism involving multiple processes. In an earlier study, we reported that the levels of serine/threonine phosphatase POPX2were positively correlated with cancer cell motility through modulating MAPK signaling. Surprisingly, here we found that POPX2 knockdown cells induced more numerous and larger tumor nodules in lungs in longer term animal studies. Interestingly, our analysis of DNA microarray data from cancer patient samples that are available on public databases shows that low POPX2 expression is linked to poor survival rate and patients with distant metastasis.These observations suggest that lower levels of POPX2 may favor tumor progression in later stages of metastasis.We hypothesize that POPX2 may do so by modulation of angiogenesis. Secretomeanalysis of POPX2-knockdown MDA-MB-231 cells using LC-MS/MS based SILAC quantitative proteomics and cytokine arrayshow that silencing of POPX2 leads to increased secretion of exosomes, which may in turn induce multiple pro-angiogenic cytokines.This study, combined with our earlier findings,suggeststhat a single ubiquitously expressed phosphatase POPX2 influences cancer metastasisvia modulating multiple biological processes including MAPK signaling and exosome-cytokine secretion.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:04:47.810.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Songjing Zhang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	6x(13)C labeled residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-04-12 02:11:50	ID requested
1	2017-01-02 07:24:34	announced
2	2017-10-24 04:13:09	announced	Updated project metadata.
⏵ 3	2024-10-22 04:04:49	announced	2024-10-22: Updated project metadata.

10.1021/acs.jproteome.6b00748;

Zhang S, Weng T, Cheruba E, Guo T, Chan H, Sze SK, Koh CG, Phosphatase POPX2 Exhibits Dual Regulatory Functions in Cancer Metastasis. J Proteome Res, 16(2):698-711(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: POPX2 phosphatase, exosome, cytokine,Tumor angiogenesis, SILAC mass spectrometry, secretome

Cheng Gee Koh
contact affiliation	Nanyang Technological University School of Biological Science
contact email	cgkoh@ntu.edu.sg
lab head
Songjing Zhang
contact affiliation	National University of Singapore
contact email	MBIZHAN@nus.edu.sg
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/01/PXD003940

PRIDE project URI

• PRIDE
  1. PXD003940
     1. Label: PRIDE project
     2. Name: Phosphatase POPX2 exhibits dual regulatory functions in cancer metastasis