PXD003936 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic profiling of VCP substrates links VCP to K6-linked ubiquitylation and c-Myc function |
Description | VCP is an evolutionary conserved ubiquitin-dependent ATPase that mediates the degradation of proteins through the ubiquitin-proteasome pathway. Despite the central role of VCP in the regulation of protein homeostasis, identity and nature of its cellular substrates remain poorly defined. Here, we combined chemical inhibition of VCP and quantitative ubiquitin remnant profiling to assess the effect of VCP inhibition on the ubiquitin-modified proteome and to probe the substrate spectrum of VCP in human cells. We demonstrate that inhibition of VCP perturbs cellular ubiquitylation and increases ubiquitylation of a different subset of proteins compared to proteasome inhibition. VCP inhibition globally upregulates K6-linked ubiquitylation that is dependent on the HECT-type ubiquitin E3 ligase HUWE1. We report ~450 putative VCP substrates, many of which function in nuclear processes, including gene expression, DNA repair and cell cycle. Moreover, we identify that VCP regulates the level and activity of the transcription factor c-Myc.
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HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:04:44.351.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Petra Beli |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | ubiquitinylated lysine |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-04-10 23:54:48 | ID requested | |
1 | 2018-02-09 11:29:37 | announced | |
2 | 2018-02-14 02:01:01 | announced | Updated project metadata. |
3 | 2019-02-12 08:07:14 | announced | Updated project metadata. |
⏵ 4 | 2024-10-22 04:04:52 | announced | 2024-10-22: Updated project metadata. |
Publication List
Heidelberger JB, Voigt A, Borisova ME, Petrosino G, Ruf S, Wagner SA, Beli P, Proteomic profiling of VCP substrates links VCP to K6-linked ubiquitylation and c-Myc function. EMBO Rep, 19(4):(2018) [pubmed] |
10.15252/embr.201744754; |
Keyword List
curator keyword: Biological |
submitter keyword: ubiquitin-proteasome system, ubiquitin remnant profiling, quantitative mass spectrometry,Protein homeostasis, VCP |
Contact List
Petra Beli |
contact affiliation | Institute of Molecular Biology (IMB), Mainz, Germany |
contact email | p.beli@imb-mainz.de |
lab head | |
Petra Beli |
contact affiliation | Institute of Molecular Biology (IMB), Mainz, Germany |
contact email | p.beli@imb-mainz.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003936
- Label: PRIDE project
- Name: Proteomic profiling of VCP substrates links VCP to K6-linked ubiquitylation and c-Myc function