PXD003925

PXD003925 is an original dataset announced via ProteomeXchange.

Title	Spatial and temporal proteomic of HCMV-infected cells
Description	Project for the study of spatial and temporal alterations in organelle proteins during HCMV infection. Two datasets are included, one for label-free quantification and one for TMT quantification. Samples were collected at 5 time points of infection (24, 48, 72, 96, and 120 hours) and subject to organelle fractionation. One non-infected sample was included for the label-free analysis, and 5 non-infected samples (one per time point) were included for the TMT dataset. The nucleus and cytosolic fractions were removed by differential centrifugation, leaving a crude organelle fraction. The crude organelle fraction was further fractionated by density gradient ultracentrifugation into sicx fractions.
HostingRepository	PRIDE
AnnounceDate	2016-09-27
AnnouncementXML	Submission_2016-09-27_05:24:28.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pierre Jean Beltran
SpeciesList	scientific name: Human cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5); NCBI TaxID: 10360; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue
Instrument	LTQ Orbitrap Velos; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-04-07 12:21:49	ID requested
⏵ 1	2016-09-27 05:24:29	announced

Jean Beltran PM, Mathias RA, Cristea IM, A Portrait of the Human Organelle Proteome In Space and Time during Cytomegalovirus Infection. Cell Syst, 3(4):361-373.e6(2016) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: HCMV spatial organelle temporal cytomegalovirus fibroblasts

Ileana Cristea
contact affiliation	Molecular Biology Department, Princeton University, Princeton, USA
contact email	icristea@princeton.edu
lab head
Pierre Jean Beltran
contact affiliation	Princeton University
contact email	pierrej@princeton.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/09/PXD003925

PRIDE project URI

• PRIDE
  1. PXD003925
     1. Label: PRIDE project
     2. Name: Spatial and temporal proteomic of HCMV-infected cells