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PXD003924

PXD003924 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleNanonets collect cancer secretome from pericellular space
DescriptionIdentifying novel cancer biomarkers is important for early cancer detection as it can reduce mortality rates. The cancer sectretome, the collection of all macromolecules secreted by a tumor cell, alters its composition compared to normal tissue, and this change plays an important role in the observation of cancer progression. The collection and accurate analysis of cancer secretomes could lead to the discovery of novel biomarkers, thus improving outcomes of cancer treatment. We unexpectedly discovered that enzyme-instructed self-assembly (EISA) of a D-peptide hydrogelator results in nanonets/hydrogel around cancer cells that overexpress ectophosphatases. Here we show that these nanonets are able to rapidly collect proteins in the pericellular space (i.e., near the surface) of cancer cells. Because the secretory substances are at their highest concentration near the cell surface, the use of pericellular nanonets to collect the cancer secretome maximizes the yield and quality of samples, reduces pre-analytical variations, and allows the dynamic profiling of secretome samples. Thus, this new approach has great potential in identifying the heterotypic signaling in tumor microenvironments thereby improving the understanding of tumor microenvironments and accelerating the discovery of potential biomarkers in cancer biology.
HostingRepositoryPRIDE
AnnounceDate2016-04-27
AnnouncementXMLSubmission_2016-04-27_01:34:01.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD003924
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterRong Zhou
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide - site C: 57.021464
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-04-07 12:08:38ID requested
12016-04-27 01:34:03announced
Publication List
Zhou R, Kuang Y, Zhou J, Du X, Li J, Shi J, Haburcak R, Xu B, Nanonets Collect Cancer Secretome from Pericellular Space. PLoS One, 11(4):e0154126(2016) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Human, Secretome, Cancer
Contact List
Bing Xu
contact affiliationDepartment of Chemistry, Brandeis University, US
contact emailbxu@brandeis.edu
lab head
Rong Zhou
contact affiliationBrandeis University
contact emailzhourong@brandeis.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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