PXD003923

PXD003923 is an original dataset announced via ProteomeXchange.

Title	Identification of A. thaliana 2-Cys peroxiredoxins interacting proteins
Description	Peroxiredoxins are ubiquitous thiol-dependent peroxidases for which chaperone and signaling roles have been reported in various types of organisms in the last years. In plants, the peroxidase function of the two typical plastidial 2-Cys peroxiredoxins (2-Cys PRX A and B) has been highlighted while the other functions, particularly in ROS-dependent signal transduction pathways, are still elusive due notably to the lack of knowledge on interacting partners. Using an ex vivo approach based on co-immunoprecipitation of leaf extracts from Arabidopsis thaliana wild-type and mutant plants lacking 2-Cys PRX expression followed by mass spectrometry-based proteomics, 158 proteins were found associated to 2-Cys PRXs, notably already known partners like thioredoxin-related electron donors (Chloroplastic Drought-induced Stress Protein of 32 kDa, Atypical Cysteine Histidine rich Thioredoxin 2) and enzymes involved in chlorophyll synthesis (Protochlorophyllide OxidoReductase B) or carbon metabolisms (Fructose-1,6-BisPhosphatase), validating the relevance of this approach.
HostingRepository	PRIDE
AnnounceDate	2016-10-11
AnnouncementXML	Submission_2016-10-11_02:31:46.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003923
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yohann Couté
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-04-07 06:42:00	ID requested
⏵ 1	2016-10-11 02:31:47	announced

Cerveau D, Kraut A, Stotz HU, Mueller MJ, Cout, é Y, Rey P, Characterization of the Arabidopsis thaliana 2-Cys peroxiredoxin interactome. Plant Sci, 252():30-41(2016) [pubmed]

curator keyword: Biological

submitter keyword: A. thaliana, peroxiredoxins, interactome, nanoLC-MS/MS

Christophe Bruley
contact affiliation	EDyP
contact email	christophe.bruley@cea.fr
lab head
Yohann Couté
contact affiliation	Laboratoire d'Etude de la Dynamique des Protéomes
contact email	yohann.coute@cea.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003923
     1. Label: PRIDE project
     2. Name: Identification of A. thaliana 2-Cys peroxiredoxins interacting proteins