PXD003913

PXD003913 is an original dataset announced via ProteomeXchange.

Title	Identification of human PKD2 interaction partners from Golgi-enriched protein fractions of HeLa cells by affinity enrichment cross-linking/MS
Description	We investigated the interaction network of human PKD2 in the cytosol as well as in Golgi-enriched subcellular protein fractions, using an affinity enrichment strategy combined with chemical cross-linking/mass spectrometry (MS). Analysis of the subproteomes revealed the presence of distinct proteins in the cytosolic and Golgi fractions. The covalent fixation of transient or weak interactors by chemical cross-linking allowed capturing interaction partners that might otherwise vanish during conventional pull-down experiments. In total, 31 interaction partners were identified for PKD2.
HostingRepository	PRIDE
AnnounceDate	2016-09-27
AnnouncementXML	Submission_2016-09-27_12:22:22.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Björn Häupl
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2016-04-06 00:06:57	ID requested
⏵ 1	2016-09-27 12:22:24	announced

H, ä, upl B, Ihling CH, Sinz A, Protein Interaction Network of Human Protein Kinase D2 Revealed by Chemical Cross-Linking/Mass Spectrometry. J Proteome Res, 15(10):3686-3699(2016) [pubmed]

curator keyword: Technical, Biological

submitter keyword: Human, PKD2, HeLa cells, Golgi apparatus, Affinity enrichment, Cross-linking/MS, Orbitrap Fusion

Andrea Sinz
contact affiliation	Department of Pharmaceutical Chemistry and Bioanalytics Institute of Pharmacy Martin Luther University Halle-Wittenberg Halle (Saale), Germany
contact email	andrea.sinz@pharmazie.uni-halle.de
lab head
Björn Häupl
contact affiliation	Martin Luther University Halle-Wittenberg
contact email	bjoern.haeupl@pharmazie.uni-halle.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003913
     1. Label: PRIDE project
     2. Name: Identification of human PKD2 interaction partners from Golgi-enriched protein fractions of HeLa cells by affinity enrichment cross-linking/MS