PXD003912

PXD003912 is an original dataset announced via ProteomeXchange.

Title	New alterations of the platelet proteome in type I Glanzmann thrombasthenia
Description	The project contains raw and result files of a proteomics analysis of two patients with type 1 Glanzmann thrombasthenia (Patient1 and 2) caused by homozygous ITGA2b delG mutations and from family (heterozygous: Father1, Mother1, Sister1, healthy: Sister2) and unrelated controls. A label-free analysis has been conducted in duplicates using Progenesis (NonLinear Dynamics) demonstrating that less than 5% of glycoprotein IIb and 5-7% fibrinogen is expressed compared to healthy controls. Heterozygous familiy members from one of the controls expressed 50-60% of GPIIb sufficient for normal GPIIb/IIIa-dependant platelet function. In a subsequent targeted LC-MS/MS analysis we quantified 14 protein identified as potentially regulated in preliminary iTRAQ experiments. We observed diminished expession of factor XIIIB chain (P05160), plasminogen (P00747) and carboxypeptidase 2B (Q96IY4) in thrombasthenic platelets as well as a 2.5-fold increased expression of FcgRIIA (P12318/P31995) and laminin alpha4 (Q16363).
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:05:11.146.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stefan Loroch
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-04-06 00:05:51	ID requested
1	2019-01-14 07:53:51	announced
⏵ 2	2024-10-22 04:05:12	announced	2024-10-22: Updated project metadata.

10.1160/th16-07-0515;

Loroch S, Trabold K, Gambaryan S, Rei, ß C, Schwierczek K, Fleming I, Sickmann A, Behnisch W, Zieger B, Zahedi RP, Walter U, Jurk K, Alterations of the platelet proteome in type I Glanzmann thrombasthenia caused by different homozygous delG frameshift mutations in ITGA2B. Thromb Haemost, 117(3):556-569(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: Glanzmann thrombasthenia, LC-MS,Platelets, Proteomics

Albert Sickmann
contact affiliation	Leibniz-Institut für Analytische Wissenschaften - ISAS - e.V. Otto-Hahn-Strasse 6b 44227 Dortmund Germany
contact email	sickmann@isas.de
lab head
Stefan Loroch
contact affiliation	Leibniz-Institut für Analytische Wissenschaften, Otto-Hahn-Straße 6b, 44227 Dortmund
contact email	stefan.loroch@isas.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD003912

PRIDE project URI

• PRIDE
  1. PXD003912
     1. Label: PRIDE project
     2. Name: New alterations of the platelet proteome in type I Glanzmann thrombasthenia