PXD003912 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | New alterations of the platelet proteome in type I Glanzmann thrombasthenia |
Description | The project contains raw and result files of a proteomics analysis of two patients with type 1 Glanzmann thrombasthenia (Patient1 and 2) caused by homozygous ITGA2b delG mutations and from family (heterozygous: Father1, Mother1, Sister1, healthy: Sister2) and unrelated controls. A label-free analysis has been conducted in duplicates using Progenesis (NonLinear Dynamics) demonstrating that less than 5% of glycoprotein IIb and 5-7% fibrinogen is expressed compared to healthy controls. Heterozygous familiy members from one of the controls expressed 50-60% of GPIIb sufficient for normal GPIIb/IIIa-dependant platelet function. In a subsequent targeted LC-MS/MS analysis we quantified 14 protein identified as potentially regulated in preliminary iTRAQ experiments. We observed diminished expession of factor XIIIB chain (P05160), plasminogen (P00747) and carboxypeptidase 2B (Q96IY4) in thrombasthenic platelets as well as a 2.5-fold increased expression of FcgRIIA (P12318/P31995) and laminin alpha4 (Q16363). |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:05:11.146.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Stefan Loroch |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos; Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-04-06 00:05:51 | ID requested | |
1 | 2019-01-14 07:53:51 | announced | |
⏵ 2 | 2024-10-22 04:05:12 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1160/th16-07-0515; |
Loroch S, Trabold K, Gambaryan S, Rei, ß C, Schwierczek K, Fleming I, Sickmann A, Behnisch W, Zieger B, Zahedi RP, Walter U, Jurk K, Alterations of the platelet proteome in type I Glanzmann thrombasthenia caused by different homozygous delG frameshift mutations in ITGA2B. Thromb Haemost, 117(3):556-569(2017) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: Glanzmann thrombasthenia, LC-MS,Platelets, Proteomics |
Contact List
Albert Sickmann |
contact affiliation | Leibniz-Institut für Analytische Wissenschaften - ISAS - e.V. Otto-Hahn-Strasse 6b 44227 Dortmund Germany |
contact email | sickmann@isas.de |
lab head | |
Stefan Loroch |
contact affiliation | Leibniz-Institut für Analytische Wissenschaften, Otto-Hahn-Straße 6b, 44227 Dortmund |
contact email | stefan.loroch@isas.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003912
- Label: PRIDE project
- Name: New alterations of the platelet proteome in type I Glanzmann thrombasthenia