PXD003904

PXD003904 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Application of 193 nm UVPD for phosphoproteomic analysis of HeLa and HCC70
Description	Application of 193 nm ultraviolet photodissociation (UVPD) for phosphoproteomics analysis of IMAC enriched HeLa and HCC70 lysates. HCC70 cells were either maintained in full media or serum starved for 6 hours prior to lysis. Each sample was analyzed by three UVPD and three HCD technical replicates using an Orbitrap Fusion mass spectrometer equipped with a Coherent 193 nm excimer laser. Using UVPD, a/x, b/y, and c/z ions were generated and improved phosphate retention was observed on product ions.
HostingRepository	MassIVE
AnnounceDate	2017-02-23
AnnouncementXML	Submission_2017-02-23_07:43:05.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michelle Robinson
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Phospho
Instrument	Orbitrap Fusion ETD

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2016-04-04 20:44:46	ID requested
⏵ 1	2017-02-23 07:43:06	announced

Publication List

Robinson MR, Taliaferro JM, Dalby KN, Brodbelt JS, 193 nm Ultraviolet Photodissociation Mass Spectrometry for Phosphopeptide Characterization in the Positive and Negative Ion Modes. J Proteome Res, 15(8):2739-48(2016) [pubmed]

Robinson MR, Taliaferro JM, Dalby KN, Brodbelt JS, 193 nm Ultraviolet Photodissociation Mass Spectrometry for Phosphopeptide Characterization in the Positive and Negative Ion Modes. J Proteome Res, 15(8):2739-48(2016) [pubmed]

Keyword List

submitter keyword: Phosphoproteomics, UVPD, HCD, HeLa, HCC70

submitter keyword: Phosphoproteomics, UVPD, HCD, HeLa, HCC70

Contact List

principal_investigator
lab head
Michelle Robinson
contact affiliation	UT Austin
contact email	mrrobinson@cm.utexas.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000079626