PXD003901

PXD003901 is an original dataset announced via ProteomeXchange.

Title	20160330_Lentinus crinitus Secretome
Description	The analysis of fungi secreted proteins has been increasingly employed as a powerful strategy in the prospecting of new catalysts with potential biotechnological application. Since enzyme production is strongly modulated by several factors such as pH, available nutrients, water content, oxygen levels and temperature, the evaluation of growth conditions is of utmost importance to achieve optimal enzyme production. Here, a non-sequenced wood-rotting fungus, L. crinitus, was selected as a model organism for secretome analysis by means of in vitro enzymatic assays and in-depth characterization via proteomics. For enzyme production, the fungus was cultured with several types and concentrations of carbon and nitrogen sources and variable water content. Five different carbon substrates (glucose, maltose, starch, sucrose, carboxymethylcellulose (CMC), glycerol and fructose) and three nitrogen containing compounds (urea, sodium nitrate and ammonium chloride) were used as substitutes of the original carbon and nitrogen sources in culture media. Interestingly, the biomass yield as well as the array of secreted proteins differed drastically under different growing conditions. A mixture of soluble secreted extracts derived from different culture conditions was analyzed both by shotgun mass spectrometry and through protein separation by two-dimensional gel electrophoresis (2DE) prior to identification via LC-MS/MS. Proteins were identified by sequence homology searches using mass spectrometry (MS)-driven BLAST. The spectrum of secreted enzymes comprised various types of CAZymes (carbohydrate-active enzymes), oxidase/reductases, proteases, lipase/esterases, proteins with non-related functions thereby classified as miscellany proteins and hypothetical or unknown predicted proteins. Although pre-separation by 2DE improved the number of identifications (protein map of 150 spots corresponding to 171 identifications) compared to the shotgun approach (98 identifications) both strategies revealed similar distribution of proteins within the functional categories described above. Culture media with reduced water content stimulated the expression of oxidases/reductases such as Lac, MnP, GMC oxidoreductases and glyoxal oxidase while hydrolases were induced during submerged fermentation. The diversity of proteins observed within both the CAZyme and oxidoreductase groups revealed in this fungus a powerful arsenal of enzymes dedicated to the breakdown and consumption of lignocellulose. Moreover, further secretome characterization after sequencing and analysis of the L. crinitus genome can potentially lead to the discovery of novel enzymes of industrial and biotechnological interest. Importantly, information on sequencing data could also reveal novel enzymes which production is strongly stimulated only in specific growing conditions.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:05:11.966.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mirta Sousa
SpeciesList	scientific name: Lentinus crinitus; NCBI TaxID: 5358;
ModificationList	S-carboxamidoethyl-L-cysteine; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-04-04 05:35:05	ID requested
1	2017-01-03 03:13:25	announced
2	2017-10-24 03:37:45	announced	Updated project metadata.
⏵ 3	2024-10-22 04:05:15	announced	2024-10-22: Updated project metadata.

Cambri G, de Sousa MM, Fonseca DM, Marchini F, da Silveira JL, Paba J, Analysis of the Biotechnological Potential of a Lentinus crinitus Isolate in the Light of Its Secretome. J Proteome Res, 15(12):4557-4568(2016) [pubmed]

curator keyword: Biological

submitter keyword: Lentinus, 2D-PAGE, secretome, LC-MS/MS

Jaime Paba
contact affiliation	Departamento de Bioquímica Setor de Ciências Biológicas Centro Politécnico Universidade Federal do Paraná BRAZIL
contact email	jaimepaba@gmail.com
lab head
Mirta Sousa
contact affiliation	Norwegian University of Science and Technology (NTNU)
contact email	sousa@ntnu.no
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003901
     1. Label: PRIDE project
     2. Name: 20160330_Lentinus crinitus Secretome