PXD003900

PXD003900 is an original dataset announced via ProteomeXchange.

Title	The exposed proteomes of Brachyspira hyodysenteriae and B. pilosicoli
Description	Applying a shotgun proteomics approach, we built a unique database of Brachyspira hyodysenteriae and B. pilosicoli peptides and proteins. A total of 148 LC-MS/MS runs were conducted on an Orbitrap instrument, and more than 29000 different peptides in cell supernatants and partially digested cell suspensions of 3 strains of B. hyodysenteriae and 2 strains of B. pilosicoli were identified. Overall, 16970 and 15493 peptide sequences, which pointed to 1625 and 1338 different protein accessions, were identified in B. hyodysenteriae and B. pilosicoli, respectively. The large-scale characterization of peptides and proteins in the extracellular medium as well as of the exposed proteins on the bacterial surface in these species provides a unique collection of interrelated data on the molecules involved in the interaction of these bacteria with their environment.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:49:00.277.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Montserrat Carrascal
SpeciesList	scientific name: Brachyspiraceae; NCBI TaxID: 143786;
ModificationList	2-pyrrolidone-5-carboxylic acid (Glu); monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-04-04 04:14:57	ID requested
1	2016-07-05 07:21:02	announced
⏵ 2	2023-11-14 08:49:00	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Brachyspira, immunogenic proteins, exoproteome,shotgun proteomics, surfaceome

Montserrat Carrascal
contact affiliation	CSIC-UAB Proteomics Laboratory IIBB-CSIC Rosello 161, 6 planta 08036 Barcelona Spain
contact email	montserrat.carrascal.csic@uab.cat
lab head
Montserrat Carrascal
contact affiliation	IIBB-CSIC
contact email	montserrat.carrascal.csic@uab.cat
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003900

PRIDE project URI

• PRIDE
  1. PXD003900
     1. Label: PRIDE project
     2. Name: The exposed proteomes of Brachyspira hyodysenteriae and B. pilosicoli