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PXD003900

PXD003900 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe exposed proteomes of Brachyspira hyodysenteriae and B. pilosicoli
DescriptionApplying a shotgun proteomics approach, we built a unique database of Brachyspira hyodysenteriae and B. pilosicoli peptides and proteins. A total of 148 LC-MS/MS runs were conducted on an Orbitrap instrument, and more than 29000 different peptides in cell supernatants and partially digested cell suspensions of 3 strains of B. hyodysenteriae and 2 strains of B. pilosicoli were identified. Overall, 16970 and 15493 peptide sequences, which pointed to 1625 and 1338 different protein accessions, were identified in B. hyodysenteriae and B. pilosicoli, respectively. The large-scale characterization of peptides and proteins in the extracellular medium as well as of the exposed proteins on the bacterial surface in these species provides a unique collection of interrelated data on the molecules involved in the interaction of these bacteria with their environment.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:49:00.277.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMontserrat Carrascal
SpeciesList scientific name: Brachyspiraceae; NCBI TaxID: 143786;
ModificationList2-pyrrolidone-5-carboxylic acid (Glu); monohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-04-04 04:14:57ID requested
12016-07-05 07:21:02announced
22023-11-14 08:49:00announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biological
submitter keyword: Brachyspira, immunogenic proteins, exoproteome,shotgun proteomics, surfaceome
Contact List
Montserrat Carrascal
contact affiliationCSIC-UAB Proteomics Laboratory IIBB-CSIC Rosello 161, 6 planta 08036 Barcelona Spain
contact emailmontserrat.carrascal.csic@uab.cat
lab head
Montserrat Carrascal
contact affiliationIIBB-CSIC
contact emailmontserrat.carrascal.csic@uab.cat
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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