PXD003887

PXD003887 is an original dataset announced via ProteomeXchange.

Title	Black pepper proteome
Description	In this study, we have evaluated the proteomic changes that occur in Piper nigrum L.(black pepper) after infection by the pathogen Phytophthora capsici. We report novel leaf proteins from black pepper identified by an integrated transcriptome-assisted label-free quantitative proteomics pipeline. Several previously described methods were used to create this data set. Detached leaves were inoculated with either mock treatment, or the oomycete pathogen and small tissue samples only around the site of inoculation were collected for protein sample preparations. In order to quantify protein abundance in the samples being compared, we used a label free method of spiking samples with a known ratio of pre-digested peptide samples to normalize endogenous protein abundance in the MS detection. Our study attempts to explain the basal immune components of black pepper when challenged with P. capsici.
HostingRepository	PRIDE
AnnounceDate	2017-11-17
AnnouncementXML	Submission_2019-05-03_07:44:14.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003887
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Chidambareswaren Mahadevan
SpeciesList	scientific name: Piper nigrum; NCBI TaxID: 13216;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2016-04-01 02:02:49	ID requested
⏵ 1	2019-05-03 07:44:15	announced

Mahadevan C, Krishnan A, Saraswathy GG, Surendran A, Jaleel A, Sakuntala M, Transcriptome- Assisted Label-Free Quantitative Proteomics Analysis Reveals Novel Insights into Piper nigrum-Phytophthora capsici Phytopathosystem. Front Plant Sci, 7():785(2016) [pubmed]

curator keyword: Biological

submitter keyword: Piper nigrum, PLGS, label-free quantitative proteomics,

Manjula Sakuntala
contact affiliation	Rajiv Gandhi Center for Biotechnology, Thiruvanthapuram, India
contact email	smanjula@rgcb.res.in
lab head
Chidambareswaren Mahadevan
contact affiliation	Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala
contact email	chidambareswaren@rgcb.res.in
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD003887

PRIDE project URI

• PRIDE
  1. PXD003887
     1. Label: PRIDE project
     2. Name: Black pepper proteome