PXD003884

PXD003884 is an original dataset announced via ProteomeXchange.

Title	Peptidomics of Acanthoscurria gomesiana spider venom reveals new toxins with potential antimicrobial activity
Description	Acanthoscurria gomesiana is a Brazilian spider from the Theraphosidae family inhabiting regions of Southeastern Brazil. Potent antimicrobial peptides as gomesin and acanthoscurrin have been discovered from the spider hemolymph in previous works. Spider venoms are also recognized as sources of biologically active peptides, however the venom peptidome of A. gomesiana remained unexplored to date. In this work, a MS-based workflow was applied to the investigation of the spider venom peptidome. Data-independent and data-dependent LC-MS/MS acquisitions of intact peptides and of peptides submitted to multiple enzyme digestions, followed by automated chromatographic alignment, de novo analysis, database and homology searches with manual validations showed that the venom is composed by less than 165 features, with masses ranging from 0.4-15.8 kDa. A total of 135 peptides from 17 proteins were identified, including three new mature peptides: U1-TRTX-Agm1a, U1-TRTX-Agm2a and U1-TRTX-Agm3a, containing 3, 4 and 3 disulfide bonds, respectively. U1-TRTX-Agm1a differed by only one amino acid from U1-TRTX-Ap1a from A. paulensis and U1-TRTX-Agm2a was derived from the genicutoxin-D1 precursor from A. geniculata. These toxins have potential applications as antimicrobial agents, as the peptide fraction of A. gomesiana showed activity against Escherichia coli strains.
HostingRepository	PRIDE
AnnounceDate	2016-08-02
AnnouncementXML	Submission_2016-08-02_06:24:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexandre Keiji Tashima
SpeciesList	scientific name: Acanthoscurria gomesiana (Tarantula spider) (Phormictopus pheopygus); NCBI TaxID: 115339;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-03-31 01:10:15	ID requested
⏵ 1	2016-08-02 06:24:45	announced

Abreu TF, Sumitomo BN, Nishiyama MY, Oliveira UC, Souza GH, Kitano ES, Zelanis A, Serrano SM, Junqueira-de-Azevedo I, Silva PI, Tashima AK, Peptidomics of Acanthoscurria gomesiana spider venom reveals new toxins with potential antimicrobial activity. J Proteomics, 151():232-242(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Acanthoscurria gomesiana, peptidomics, antimicrobial peptide, theraphotoxin.

Alexandre Keiji Tashima
contact affiliation	Departamento de Bioquimica, Escola Paulista de Medicina, Universidade Federal de Sao Paulo, SP, Brazil
contact email	aktashima@unifesp.br
lab head
Alexandre Keiji Tashima
contact affiliation	Universidade Federal de Sao Paulo
contact email	aktashima@unifesp.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003884
     1. Label: PRIDE project
     2. Name: Peptidomics of Acanthoscurria gomesiana spider venom reveals new toxins with potential antimicrobial activity