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PXD003882

PXD003882 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDrosophila mRNA Interactome - Global changes of the RNA-bound proteome during the maternal-to-zygotic transition in Drosophila
DescriptionThe maternal-to-zygotic transition (MZT) is a process that occurs in animal embryos at the earliest developmental stages, during which maternally deposited mRNAs and other molecules are degraded and replaced by products of the zygotic genome. The zygotic genome is not activated immediately upon fertilization, therefore post-transcriptional mechanisms control the first steps of development in the early, pre-MZT embryo. To perform unbiased organism-wide identification of Drosophila RNA binding proteins (RPBs), crucial players of post-transcriptional control, we applied the recently developed RNA interactome capture method, which involves cross-linking of RNAs and their direct protein partners by UV light, purification of RNA under stringent conditions and identification of proteins by mass spectrometry. Our analysis yielded 523 high confidence RBP hits, half of which were not previously reported to bind RNA. Our comparison of the RNA interactomes of pre- and post-MZT embryos reveals a highly dynamic behavior of the RNA-bound proteome during early development, and suggests active regulation of RNA binding of some RBPs. This resource provides the first evidence of RNA binding for hundreds of Drosophila proteins, and opens new avenues for study of molecular mechanisms of early development.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:03:52.070.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChristian Frese
SpeciesList scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-03-31 00:32:29ID requested
12016-07-11 06:16:54announced
22024-10-22 04:03:56announced2024-10-22: Updated project metadata.
Publication List
10.1038/ncomms12128;
Sysoev VO, Fischer B, Frese CK, Gupta I, Krijgsveld J, Hentze MW, Castello A, Ephrussi A, Global changes of the RNA-bound proteome during the maternal-to-zygotic transition in Drosophila. Nat Commun, 7():12128(2016) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: TMT-MS3,Drosophila development, RNA-binding proteins
Contact List
Jeroen Krijgsveld
contact affiliationDKFZ German Cancer Research Center Proteomics of Stem Cells and Cancer (B230) Im Neuenheimer Feld 581 69120 Heidelberg, Germany
contact emailj.krijgsveld@dkfz-heidelberg.de
lab head
Christian Frese
contact affiliationMax Planck Unit for the Science of Pathogens
contact emailfrese@mpusp.mpg.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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