PXD003879

PXD003879 is an original dataset announced via ProteomeXchange.

Title	The response of the root proteome to the synthetic strigolactone GR24 in Arabidopsis
Description	Strigolactones are plant metabolites that act as phytohormones and rhizosphere signals. Whereas most research on unraveling the action mechanisms of strigolactones is focused on plant shoots, we investigated proteome adaptation during strigolactone signaling in the roots of Arabidopsis thaliana. Through large-scale, time-resolved, and quantitative proteomics, the impact of the strigolactone analog rac-GR24 was elucidated on the root proteome of the wild type and the signaling mutant more axillary growth 2 (max2). Our study revealed a clear MAX2-dependent rac-GR24 response: an increase in abundance of enzymes involved in flavonol biosynthesis, which was reduced in the max2-1 mutant. Mass spectrometry-driven metabolite profiling and thin-layer chromatography experiments demonstrated that these changes in protein expression lead to the accumulation of specific flavonols. Moreover, quantitative RT-PCR revealed that the flavonol-related protein expression profile was caused by rac-GR24--induced changes in transcript levels of the corresponding genes. This induction of flavonol production was shown to be activated by the two pure enantiomers that together make up rac-GR24. Finally, our data provide much needed clues concerning the multiple roles played by MAX2 in the roots and a comprehensive view of the rac-GR24--induced response in the root proteome.
HostingRepository	PRIDE
AnnounceDate	2016-06-20
AnnouncementXML	Submission_2016-06-20_04:18:38.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	lukas Braem
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-03-30 08:04:37	ID requested
⏵ 1	2016-06-20 04:18:39	announced

Walton A, Stes E, Goeminne G, Braem L, Vuylsteke M, Matthys C, De Cuyper C, Staes A, Vandenbussche J, Boyer FD, Vanholme R, Fromentin J, Boerjan W, Gevaert K, Goormachtig S, The Response of the Root Proteome to the Synthetic Strigolactone GR24 in Arabidopsis. Mol Cell Proteomics, 15(8):2744-55(2016) [pubmed]

submitter keyword: Root responses to strigolactone treatment

Kris Gevaert
contact affiliation	MBC - VIB/UGENT
contact email	kris.gevaert@vib-ugent.be
lab head
lukas Braem
contact affiliation	VIB
contact email	lubra@psb.vib-ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/06/PXD003879

PRIDE project URI

• PRIDE
  1. PXD003879
     1. Label: PRIDE project
     2. Name: The response of the root proteome to the synthetic strigolactone GR24 in Arabidopsis