PXD003875

PXD003875 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics analysis of exosomes purified from serum of patients with colorectal cancer
Description	Tumor cells of colorectal cancer (CRC), release exosomes into peripheral blood. These exosomes can mediate communication between cells and affect various tumor-related processes in their target cells. We present a quantitative proteomics analysis on the exosomes purified from serum of patients with CRC and normal volunteers. 918 proteins were identified with an overlap of 725 Gene IDs in Exocarta proteins list. Comparing with the serum-purified exosomes of normal volunteers, we found 36 proteins upregulated and 22 proteins downregulated in the serum-purified exosomes of CRC patients, of which over 50% were involved in protein metabolism and immune response. Pathway and interaction network analysis results revealed that most of the exosome-enriched differentially expressed proteins (DEPs) were associated with complement and coagulation cascades and cancer pathways, suggesting their potential roles in tumor carcinogenesis. Our study demonstrates that serum-purified exosomes of CRC patients can play a pivotal role in invasiveness and pre-metastatic niche establishment of CRC, but not sustain the tumor survival and proliferation. The content selection of exosomes might be associated with the functions of proteins in the development of CRC. This information will be helpful in elucidating the pathophysiological functions of tumor-derived exosomes, and aid in the development of colorectal cancer diagnostics and therapeutics.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:03:46.673.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wei Ge
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-03-30 07:56:07	ID requested
1	2016-12-12 14:17:23	announced
2	2017-10-24 02:20:29	announced	Updated project metadata.
⏵ 3	2024-10-22 04:03:51	announced	2024-10-22: Updated project metadata.

10.1002/ijc.30496;

Chen Y, Xie Y, Xu L, Zhan S, Xiao Y, Gao Y, Wu B, Ge W, Protein content and functional characteristics of serum-purified exosomes from patients with colorectal cancer revealed by quantitative proteomics. Int J Cancer, 140(4):900-913(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: proteomics,colorectal,exosome

Wei Ge
contact affiliation	National Key Laboratory of Medical Molecular Biology & Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Dongdan Santiao 5 #, Dongcheng district, Beijing 100005, China.
contact email	wei.ge@chem.ox.ac.uk
lab head
Wei Ge
contact affiliation	National Key Laboratory of Medical Molecular Biology & Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences
contact email	wei.ge@chem.ox.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/12/PXD003875

PRIDE project URI

• PRIDE
  1. PXD003875
     1. Label: PRIDE project
     2. Name: Quantitative proteomics analysis of exosomes purified from serum of patients with colorectal cancer