PXD003872
PXD003872 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Response of podocytes to PAN - YAP-mediated mechanotransduction determines the podocyte's response to damage |
Description | Podocytes are terminally differentiated cells at the kidney filtration barrier and exposed to considerable mechanical strain. Podocyte injury causes morphological changes as a result of cytoskeletal reorganizations and failure of the filtration barrier. The transcriptional co-activators YAP/TAZ are tightly controlled through hippo signaling and responsive to mechanical cues. Here, we show that YAP is upregulated upon podocyte injury to activate YAP-dependent target genes. This activation preceded the development of proteinuria. In contrast, similar perturbations of cells in culture did not reveal increased YAP activity but showed a downregulation of YAP/TAZ activity when cells were grown on stiff surface. However, culture of cells on soft matrix or inhibition of stress fiber formation allowed recapitulation of the damage-induced YAP upregulation indicating a mechanotransduction-dependent mechanism of YAP over-activity. Interestingly, increased expression of YAP targets was confirmed in renal biopsies from patients with glomerular disease. Consistently, pharmacological inhibition of YAP/TEAD activity ameliorated glomerular disease in vivo. These data suggest that perturbation of the mechanosensitive hippo signaling pathway may be a therapeutic principle in podocyte disease. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:03:47.102.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Markus Rinschen |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2016-03-30 06:18:28 | ID requested | |
1 | 2017-04-19 05:09:05 | announced | |
⏵ 2 | 2024-10-22 04:03:55 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1126/scisignal.aaf8165; |
Rinschen MM, Grahammer F, Hoppe AK, Kohli P, Hagmann H, Kretz O, Bertsch S, H, ö, hne M, G, ö, bel H, Bartram MP, Gandhirajan RK, Kr, ü, ger M, Brinkkoetter PT, Huber TB, Kann M, Wickstr, ö, m SA, Benzing T, Schermer B, YAP-mediated mechanotransduction determines the podocyte's response to damage. Sci Signal, 10(474):(2017) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Human podocytes PAN |
Contact List
Bernhard Schermer, Markus Rinschen | |
---|---|
contact affiliation | University Hospital Cologne, CECAD |
contact email | bernhard.schermer@uk-koeln.de |
lab head | |
Markus Rinschen | |
contact affiliation | Kidney research center cologne |
contact email | markus.rinschen@uk-koeln.de |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/04/PXD003872 |
PRIDE project URI |
Repository Record List
[ + ]