PXD003863

PXD003863 is an original dataset announced via ProteomeXchange.

Title	Differential proteome analysis of Escherichia coli grown on acetate vs. glucose
Description	Relative protein abundances of Escherichia coli growing exponentially on minimal medium with acetate or glucose as the sole carbon source were investigated in a quantitative shotgun proteome analysis with TMT6-plex isobaric tags. Peptides were separated by high resolution high/low pH 2D-LC, using an optimized fraction pooling scheme followed by mass spectrometric analysis. Quantitative data were acquired for 1,994 proteins covering 46 % of the predicted E. coli proteins and 361 differentially abundant proteins were discovered. Differences in protein abundance were observed for proteins in central carbon metabolism, in particular for proteins involved in TCA cycle and glyoxylate shunt, fatty acid metabolism, glycolysis/gluconeogenesis and anaplerotic pathways. Significant changes were observed in proteins relevant for amino acid and protein synthesis, proteins necessary to process environmental information and scavenge for a variety of alternate carbon sources.
HostingRepository	PRIDE
AnnounceDate	2016-10-11
AnnouncementXML	Submission_2016-10-11_01:24:15.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andreas Tholey
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; methylthiolated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-03-29 04:20:23	ID requested
⏵ 1	2016-10-11 01:24:16	announced

Treitz C, Enjalbert B, Portais JC, Letisse F, Tholey A, Differential quantitative proteome analysis of Escherichia coli grown on acetate versus glucose. Proteomics, 16(21):2742-2746(2016) [pubmed]

curator keyword: Biological

submitter keyword: isobaric, TMT, high/low pH 2D-LC

Andreas Tholey
contact affiliation	Systematic Proteome Research & Bioanalytics, University of Kiel
contact email	a.tholey@iem.uni-kiel.de
lab head
Andreas Tholey
contact affiliation	Systematic Proteome Research & Bioanalytics, University of Kiel
contact email	a.tholey@iem.uni-kiel.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003863
     1. Label: PRIDE project
     2. Name: Differential proteome analysis of Escherichia coli grown on acetate vs. glucose