PXD003861

PXD003861 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	MudPIT analysis of HIV-1 Vpr-asssociated proteins from the permissive CEM.SS human T4-lymphoblastoid cell line
Description	CEM.SS-iH1.Vpr cells expressing a tandem HA-FLAG- epitope-tagged HIV-1 NL4-3 Vpr (hfa-H.Vpr) under control of the Lenti-X Tet-On 3G Inducible Expression System (Clontech), and control CEM.SS cells (3x105 cells/ml, 4 liters), were induced with doxycycline (0.1ug/ml) for 9 hours. Whole cell extracts were prepared and tandem affinity purifications of Vpr and its associated proteins, from 5 grams of wet cell mass, were performed as described (Hrecka K, et al. Nature 2011; 474(7353):658-661). The Vpr-protein complexes and negative controls purified from T cells were digested with endoproteinase LysC followed by trypsin and the resulting peptide mixtures were analyzed by Multidimensional Protein Identification Technology (MudPIT) on an LTQ ion trap mass spectrometer as described (Florens L, Washburn MP. Methods Mol Biol 2006;328:159-75).
HostingRepository	MassIVE
AnnounceDate	2016-06-22
AnnouncementXML	Submission_2016-06-22_13:04:01.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laurence Florens
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: HIV-1 vector pNL4-3; NCBI TaxID: 151458;
ModificationList	S-carboxamidomethyl-L-cysteine
Instrument	LTQ

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2016-03-24 11:22:07	ID requested
⏵ 1	2016-06-22 13:04:02	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: HIV accessory virulence protein Vpr, affinity purification, T cells

submitter keyword: HIV accessory virulence protein Vpr, affinity purification, T cells

Contact List

Laurence FLorens
lab head
Laurence Florens
contact affiliation	Stowers Institute for Medical Research
contact email	laf@stowers.org
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000079605