PXD003860

PXD003860 is an original dataset announced via ProteomeXchange.

Title	Impact of cathepsin A on the murine cardiac proteome
Description	Cathepsin A (CTSA) is a lysosomal carboxypeptidase present at the cell surface and secreted outside the cell. Additionally, CTSA binds to β-galactosidase and neuraminidase 1 to protect them from degradation. CTSA has gained attention as a drug target for the treatment of cardiac hypertrophy and heart failure. Here we investigated the impact of CTSA on the murine cardiac proteome in a mouse model of cardiomyocyte-specific human CTSA overexpression, using liquid chromatography-tandem mass spectrometry in conjunction with an isotopic dimethyl labeling strategy. Statistical analysis by linear models for microarray data (limma) found > 300 significantly affected proteins; thus establishing CTSA as a key modulator of the cardiac proteome. CTSA strongly impaired the balance of the proteolytic system by up-regulating several proteases whilst down-regulating numerous protease inhibitors. Moreover, cardiomyocyte-specific human CTSA overexpression strongly reduced the levels of numerous proteins that are involved in the oxidative stress response.
HostingRepository	PRIDE
AnnounceDate	2016-07-25
AnnouncementXML	Submission_2016-07-25_00:29:47.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oliver Schilling
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-03-24 07:29:30	ID requested
⏵ 1	2016-07-25 00:29:48	announced

Petrera A, Kern U, Linz D, Gomez-Auli A, Hohl M, Gassenhuber J, Sadowski T, Schilling O, Proteomic Profiling of Cardiomyocyte-Specific Cathepsin A Overexpression Links Cathepsin A to the Oxidative Stress Response. J Proteome Res, 15(9):3188-95(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: cathepsin A, transgenic mouse, LC-MS/MS

Oliver Schilling
contact affiliation	Institute of Molecular Medicine and Cell Research, University of Freiburg, Stefan Meier Strasse 17, 79104, Freiburg, Germany
contact email	oliver.schilling@mol-med.uni-freiburg.de
lab head
Oliver Schilling
contact affiliation	University of Freiburg
contact email	oliver.schilling@mol-med.uni-freiburg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003860
     1. Label: PRIDE project
     2. Name: Impact of cathepsin A on the murine cardiac proteome