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PXD003856 is an original dataset announced via ProteomeXchange.

Dataset Summary
Title¬¬ZMYND8 co-localizes with NuRD on target genes and regulates poly-ADP-ribose dependent recruitment of GATAD2A/NuRD to sites of DNA damage
DescriptionThe NuRD complex is generally thought to repress transcription at both hyper- and hypomethylated regions in the genome. In addition, the complex is involved in the DNA damage response. Here, we show that ZMYND8 bridges NuRD to a number of putative DNA-binding zinc finger proteins. The ZMYND8 MYND domain directly interacts with PPPLΦ motifs in the NuRD subunit GATAD2A. Furthermore, GATAD2A and GATAD2B exclusively form homodimers and they thus define mutually exclusive NuRD subcomplexes. ZMYND8 and MBD3 share a large number of genome-wide binding sites, mostly active promoters and enhancers. Depletion of ZMYND8 does not affect NuRD occupancy genome-wide and expression of NuRD/ZMYND8 target genes in steady-state asynchronous cells. However, ZMYND8 facilitates immediate recruitment of GATAD2A/NuRD to induced sites of DNA damage in a PAR-dependent manner. These results thus show that a specific substoichiometric interaction with a NuRD subunit paralogue provides unique functionality to a distinct NuRD subcomplex.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterCornelia Spruijt
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ FT; LTQ Orbitrap Velos; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-03-24 00:43:24ID requested
12016-10-10 00:29:22announced
22016-10-18 02:21:18announcedUpdated publication reference for PubMed record(s): 27732854.
32017-06-20 05:44:16announcedUpdated project metadata.
42017-10-24 01:53:25announcedUpdated project metadata.
Publication List
Spruijt CG, Luijsterburg MS, Menafra R, Lindeboom RG, Jansen PW, Edupuganti RR, Baltissen MP, Wiegant WW, Voelker-Albert MC, Matarese F, Mensinga A, Poser I, Vos HR, Stunnenberg HG, van Attikum H, Vermeulen M, ZMYND8 Co-localizes with NuRD on Target Genes and Regulates Poly(ADP-Ribose)-Dependent Recruitment of GATAD2A/NuRD to Sites of DNA Damage. Cell Rep, 17(3):783-798(2016) [pubmed]
Keyword List
submitter keyword: ZMYND8, NuRD, epigenetics, GFP-affinity purification, SILAC, LFQ
Contact List
Michiel Vermeulen
contact affiliationMolecular Biology, RIMLS, Nijmegen, The Netherlands
contact emailmichiel.vermeulen@science.ru.nl
lab head
Cornelia Spruijt
contact affiliationRIMLS, RU, Nijmegen, The Netherlands
contact emailnellekespruijt@hotmail.com
dataset submitter
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