PXD003854 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic and Phosphoproteomic Analyses of Yeast Reveal the Global Cellular Response to Sphingolipid Depletion |
Description | Sphingolipids are essential components of eukaryotic cells with important functions in membrane biology and cellular signaling. Their levels are tightly controlled and coordinated with the abundance of other membrane lipids. How sphingolipid homeostasis is achieved is not yet well understood. Studies performed primarily in yeast showed that the phosphorylation states of several enzymes and regulators of sphingolipid synthesis are important, although a global understanding for such regulation is lacking. Here, we used high-resolution mass-spectrometry-based proteomics and phosphoproteomics, in combination with data from a chemical genetic screen, to analyze the cellular response to sphingolipid synthesis inhibition. Our dataset reveals that changes in protein phosphorylation, rather than protein abundance, dominate the response to blocking sphingolipid synthesis. We identified Ypk1 signaling as a major pathway that is activated under these conditions, and we confirmed and identified Ypk1 targets. We also revealed key aspects of the cellular response to sphingolipid deprivation, including nodes that intersect with sterol metabolism and modification of lipid transport. Our data provide a rich resource for on-going mechanistic studies of key elements of the cellular response to the depletion of sphingolipid levels and the maintenance of sphingolipid homeostasis. |
HostingRepository | PRIDE |
AnnounceDate | 2017-10-24 |
AnnouncementXML | Submission_2017-10-24_03:38:17.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | romain christiano |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | phosphorylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-03-24 00:30:06 | ID requested | |
1 | 2017-01-03 03:11:45 | announced | |
⏵ 2 | 2017-10-24 03:38:18 | announced | Updated project metadata. |
Publication List
Fr, รถ, hlich F, Olson DK, Christiano R, Farese RV, Walther TC, Proteomic and phosphoproteomic analyses of yeast reveal the global cellular response to sphingolipid depletion. Proteomics, 16(21):2759-2763(2016) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: yeast, sphingolipid depletion, phosphoproteomics, QExactive HF |
Contact List
Tobias C Walther |
contact affiliation | Tobias C. Walther Department of Genetics and Complex Diseases Harvard School of Public Health 655 Huntington Avenue Boston, MA 02115 AFFILIATION: 1 Department of Genetics and Complex Diseases, Harvard T.H. Chan School of Public Health, Boston, MA, USA 2 Department of Cell Biology, Harvard Medical School, Boston, MA, USA 3Broad Institute of MIT and Harvard, Cambridge, MA, USA 4 Howard Hughes Medical Institute, Boston, MA, USA |
contact email | twalther@hsph.harvard.edu |
lab head | |
romain christiano |
contact affiliation | Harvard School of Public Health |
contact email | rchrist@hsph.harvard.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003854
- Label: PRIDE project
- Name: Proteomic and Phosphoproteomic Analyses of Yeast Reveal the Global Cellular Response to Sphingolipid Depletion