PXD003844

PXD003844 is an original dataset announced via ProteomeXchange.

Title	Cellulose and hemicellulose decomposition by forest soil bacteria proceeds by the action of structurally variable enzymatic systems
Description	Evidence shows that bacteria contribute actively to the decomposition of cellulose and hemicellulose in forest soil; however, their role in this process is still unclear. Here we performed the screening and identification of bacteria showing potential cellulolytic activity from litter and organic soil of a temperate oak forest. The genomes of three cellulolytic isolates previously described as abundant in this ecosystem were sequenced and their proteomes were characterized during the growth on plant biomass and on microcrystalline cellulose. Pedobacter and Mucilaginibacter showed complex enzymatic systems containing highly diverse carbohydrate-active enzymes for the degradation of cellulose and hemicellulose, which were functionally redundant for endoglucanases, -glucosidases, endoxylanases, -xylosidases, mannosidases and carbohydrate-binding modules. Luteibacter did not express any glycosyl hydrolases traditionally recognized as cellulases. Instead, cellulose decomposition was likely performed by an expressed GH23 family protein containing a cellulose-binding domain. Interestingly, the presence of plant lignocellulose as well as crystalline cellulose both trigger the production of a wide set of hydrolytic proteins including cellulases, hemicellulases and other glycosyl hydrolases. Our findings highlight the extensive and unexplored structural diversity of enzymatic systems in cellulolytic soil bacteria and indicate the roles of multiple abundant bacterial taxa in the decomposition of cellulose and other plant polysaccharides.
HostingRepository	PRIDE
AnnounceDate	2016-05-02
AnnouncementXML	Submission_2016-05-02_05:24:24.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniela Zuehlke
SpeciesList	scientific name: Mucilaginibacter; NCBI TaxID: 423349; scientific name: Luteibacter; NCBI TaxID: 242605; scientific name: Pedobacter sp.; NCBI TaxID: 1411316;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-03-23 03:36:36	ID requested
⏵ 1	2016-05-02 05:24:25	announced

L, ó, pez-Mond, é, jar R, Z, ü, hlke D, Becher D, Riedel K, Baldrian P, Cellulose and hemicellulose decomposition by forest soil bacteria proceeds by the action of structurally variable enzymatic systems. Sci Rep, 6():25279(2016) [pubmed]

curator keyword: Biological

submitter keyword: forest soil bacteria, decomposition, GeLC-MS/MS

Katharina Riedel
contact affiliation	Ernst-Moritz-Arndt University Greifswald Institute of Microbiology Friedrich-Ludwig-Jahn-Strasse 15 D-17487 Greifswald, Germany
contact email	riedela@uni-greifswald.de
lab head
Daniela Zuehlke
contact affiliation	Ernst Moritz Arndt University Greifswald, Institute of Microbiology
contact email	zuehlke@uni-greifswald.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003844
     1. Label: PRIDE project
     2. Name: Cellulose and hemicellulose decomposition by forest soil bacteria proceeds by the action of structurally variable enzymatic systems