PXD003842

PXD003842 is an original dataset announced via ProteomeXchange.

Title	Influence of mutations in PPE38 on the secretome composition of M. tuberculosis
Description	What is the influence of mutations in PPE38 on the secretome composition of M. tuberculosis? Certain clinical isolates of M. tuberculosis belonging to the Beijing lineage have mutations in ppe38 and this leads to a loss of secretion of the PE_PGRS substrates and hypervirulence in a mouse model of M. tuberculosis. Culture filtrates (secretomes) of 3 strains have been collected of which strain SAWC_2088 has intact PPE38 and Strains SAWC_2135 and SAWC_2701 have mutated PPE38. The strains with mutated PPE38 were also complemented by reintroducing the corresponding genes on an integrative plasmid. Label-free single-shot proteomics was used to profile protein expression in M. tuberculosis secretomes.
HostingRepository	PRIDE
AnnounceDate	2017-10-11
AnnouncementXML	Submission_2018-01-22_03:31:34.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sander Piersma
SpeciesList	scientific name: Mycobacterium tuberculosis; NCBI TaxID: 1773;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-03-22 09:17:14	ID requested
1	2017-10-11 04:05:44	announced
⏵ 2	2018-01-22 03:31:36	announced	Updated publication reference for PubMed record(s): 29335553.

Ates LS, Dippenaar A, Ummels R, Piersma SR, van der Woude AD, van der Kuij K, Le Chevalier F, Mata-Espinosa D, Barrios-Pay, á, n J, Marquina-Castillo B, Guapillo C, Jim, é, nez CR, Pain A, Houben ENG, Warren RM, Brosch R, Hern, á, ndez-Pando R, Bitter W, Mutations in ppe38 block PE_PGRS secretion and increase virulence of Mycobacterium tuberculosis. Nat Microbiol, 3(2):181-188(2018) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Mycobacterium tuberculosis, secretome, PPE38, label-free, single-shot

Connie Ramona Jimenez
contact affiliation	OncoProteomics Laboratory, Dept of Medical Oncology, VU University Medical Center, Amsterdam, The Netherlands
contact email	c.jimenez@vumc.nl
lab head
Sander Piersma
contact affiliation	OncoProteomics Laboratory, dept of Medical Oncology, VUmc Medical Center, Amsterdam, The Netherlands
contact email	s.piersma@vumc.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/10/PXD003842

PRIDE project URI

• PRIDE
  1. PXD003842
     1. Label: PRIDE project
     2. Name: Influence of mutations in PPE38 on the secretome composition of M. tuberculosis