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DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2017-10-11
  • AnnouncementXML: Submission_2018-01-22_03:31:34.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Sander Piersma
  • Title: Influence of mutations in PPE38 on the secretome composition of M. tuberculosis
  • Description: What is the influence of mutations in PPE38 on the secretome composition of M. tuberculosis? Certain clinical isolates of M. tuberculosis belonging to the Beijing lineage have mutations in ppe38 and this leads to a loss of secretion of the PE_PGRS substrates and hypervirulence in a mouse model of M. tuberculosis. Culture filtrates (secretomes) of 3 strains have been collected of which strain SAWC_2088 has intact PPE38 and Strains SAWC_2135 and SAWC_2701 have mutated PPE38. The strains with mutated PPE38 were also complemented by reintroducing the corresponding genes on an integrative plasmid. Label-free single-shot proteomics was used to profile protein expression in M. tuberculosis secretomes.
  • SpeciesList: scientific name: Mycobacterium tuberculosis; NCBI TaxID: 1773;
  • ModificationList: monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
  • Instrument: Q Exactive

Dataset History

VersionDatetimeStatusChangeLog Entry
02016-03-22 09:17:14ID requested
12017-10-11 04:05:44announced
22018-01-22 03:31:36announcedUpdated publication reference for PubMed record(s): 29335553.

Publication List

  1. Ates LS, Dippenaar A, Ummels R, Piersma SR, van der Woude AD, van der Kuij K, Le Chevalier F, Mata-Espinosa D, Barrios-Payán J, Marquina-Castillo B, Guapillo C, Jiménez CR, Pain A, Houben ENG, Warren RM, Brosch R, Hernández-Pando R, Bitter W, Mutations in ppe38 block PE_PGRS secretion and increase virulence of Mycobacterium tuberculosis. Nat Microbiol, 3(2):181-188(2018) [pubmed]

Keyword List

  1. curator keyword: Biological, Biomedical
  2. submitter keyword: Mycobacterium tuberculosis, secretome, PPE38, label-free, single-shot

Contact List

    Connie Ramona Jimenez
    • contact affiliation: OncoProteomics Laboratory, Dept of Medical Oncology, VU University Medical Center, Amsterdam, The Netherlands
    • contact email: c.jimenez@vumc.nl
    • lab head:
    Sander Piersma
    • contact affiliation: OncoProteomics Laboratory, dept of Medical Oncology, VUmc Medical Center, Amsterdam, The Netherlands
    • contact email: s.piersma@vumc.nl
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List
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