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DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2016-10-18
  • AnnouncementXML: Submission_2016-10-18_02:42:42.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Leslie MULLER
  • Title: Tube-gel digestion: a fast and reproducible sample preparation protocol for label-free quantitative proteomics of complex biological samples
  • Description: Comparison of tube-gel protocol to stacking gel and liquid digestion for label-free extracted ion-current quantification of complex biological samples Dataset with known fold-changes (UPS1 spiked in yeast background at 25-10-5-2.5-1-0.5 fmol)
  • SpeciesList: scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
  • ModificationList: S-carboxamidoethyl-L-cysteine; monohydroxylated residue; iodoacetamide derivatized residue
  • Instrument: Q Exactive

Dataset History

VersionDatetimeStatusChangeLog Entry
02016-03-22 06:23:54ID requested
12016-10-18 02:42:43announced

Publication List

  1. Muller L, Fornecker L, Van Dorsselaer A, Cianférani S, Carapito C, Benchmarking sample preparation/digestion protocols reveals tube-gel being a fast and repeatable method for quantitative proteomics. Proteomics, 16(23):2953-2961(2016) [pubmed]

Keyword List

  1. submitter keyword: Label-free quantitative proteomics, sample preparation, tube-gel, stacking gel, liquid digestion

Contact List

    • contact affiliation: UMR 7178 - IPHC - CNRS - UdS - Laboratory of BioOrganic Mass Spectrometry - STRASBOURG, FRANCE
    • contact email: sarah.cianferani@unistra.fr
    • lab head:
    Leslie MULLER
    • contact affiliation: UMR7178 IPHC-DSA-LSMBO
    • contact email: leslie.muller@etu.unistra.fr
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
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