PXD003827

PXD003827 is an original dataset announced via ProteomeXchange.

Title	Comparative genomic, proteomic and exoproteomic analyses of three Pseudomonas spp. - part 2
Description	Bacteria that inhabit the rhizosphere of agricultural crops can have a beneficial effect on crop growth, including through the solubilisation and remineralisation of complex forms of phosphorus (P). However [1], our understanding of the bacterial proteomic response to P stress is limited. Here, exoproteomic analysis of three Pseudomonas species was performed in unison[2] with proteomic analysis of Pseudomonas putida BIRD-1 (BIRD-1) grown [3]under P replete and P deplete conditions. Comparative exoproteomics revealed marked heterogeneity in the exoproteomes of each Pseudomonas species in response to low concentrations of P. In addition to well-characterised members of the PHO regulon such as alkaline phosphatases, several previously undiscovered proteins are responsive to phosphate depletion including putative nucleases, phosphotriesterases, putative phosphonate transporters and outer membrane proteins. Moreover, in BIRD-1, mutagenesis of the master regulator, phoBR, led us to confirm the addition of several novel PHO-dependent proteins. Our data expands knowledge of the Pseudomonas PHO regulon, including species that are frequently used as bioinoculants, opening up the potential for more efficient and complete use of soil complexed P.
HostingRepository	PRIDE
AnnounceDate	2016-08-22
AnnouncementXML	Submission_2017-02-06_01:53:56.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003827
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alex Jones
SpeciesList	scientific name: Pseudomonas fluorescens (strain SBW25); NCBI TaxID: 216595;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2016-03-21 06:32:18	ID requested
1	2016-08-22 09:01:11	announced
⏵ 2	2017-02-06 01:53:57	announced	Updated publication reference for PubMed record(s): 27233093.

Lidbury ID, Murphy AR, Scanlan DJ, Bending GD, Jones AM, Moore JD, Goodall A, Hammond JP, Wellington EM, Comparative genomic, proteomic and exoproteomic analyses of three Pseudomonas strains reveals novel insights into the phosphorus scavenging capabilities of soil bacteria. Environ Microbiol, 18(10):3535-3549(2016) [pubmed]

curator keyword: Biological

submitter keyword: exoproteome, Pseudomonas

Alex Jones
contact affiliation	University of Warwick
contact email	alex.jones@warwick.ac.uk
lab head
Alex Jones
contact affiliation	University of Warwick
contact email	alex.jones@warwick.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003827
     1. Label: PRIDE project
     2. Name: Comparative genomic, proteomic and exoproteomic analyses of three Pseudomonas spp. - part 2