PXD003825

PXD003825 is an original dataset announced via ProteomeXchange.

Title	Aspergillus flavus Induced Alterations in Tear Protein Profile Reveal Pathogen-Induced Host Response to Fungal Infection
Description	Aspergillus flavus and Fusarium sp. are primary causative agents of keratitis that results in corneal tissue damage leading to vision loss. The tear proteome of control and keratitis patients was profiled and compared. A total of 1873 proteins from control and 1400 proteins from patient tear were identified by mass spectrometry. While 847 proteins were found to be glycosylated in the patient tear, only 726 were glycosylated in control tear. Some tear proteins showed alterations in their glycosylation pattern after infection. Complement system proteins, proteins specific for neutrophil extracellular traps and proteins involved in would healing were found only in the patient tear. The presence of these innate immune system proteins in patient's tear supports previous data indicating the involvement of neutrophil and complement pathways in antifungal defense. High levels of wound healing proteins in keratitis patient tear implied activation of tissue repair. The early appearance of host defense proteins and wound healing response indicates that tear proteins could be used as an early marker system for monitoring the progression of pathogenesis. Identification of negative regulators of the defense pathways in keratitis tear indicates an intricate balance of pro and anti-defense mechanisms operating in fungal infection of the eye.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_05:46:40.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Naveen Luke
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-03-21 03:41:55	ID requested
1	2016-12-13 00:48:34	announced
2	2016-12-20 07:04:54	announced	Updated publication reference for PubMed record(s): 27872886, 27789337.
3	2017-10-24 02:39:01	announced	Updated project metadata.
⏵ 4	2017-10-24 05:46:42	announced	Updated project metadata.

Kandhavelu J, Demonte NL, Namperumalsamy VP, Prajna L, Thangavel C, Jayapal JM, Kuppamuthu D, induced alterations in tear proteome: Understanding the pathogen-induced host response to fungal infection. Data Brief, 9():888-894(2016) [pubmed]

Kandhavelu J, Demonte NL, Namperumalsamy VP, Prajna L, Thangavel C, Jayapal JM, Kuppamuthu D, Aspergillus flavus induced alterations in tear protein profile reveal pathogen-induced host response to fungal infection. J Proteomics, 152():13-21(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: mycotic keratitis

tear proteomics

complement

NETosis

wound healing

glycosylation

Dharmalingam Kuppamuthu
contact affiliation	Director-Research, Aravind Medical Research Foundation, Madurai, Tamil Nadu, India
contact email	dharmalingam.k@gmail.com
lab head
Naveen Luke
contact affiliation	Aravind Medical Research Foundation, Madurai
contact email	naveen.luke@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/12/PXD003825

PRIDE project URI

• PRIDE
  1. PXD003825
     1. Label: PRIDE project
     2. Name: Aspergillus flavus Induced Alterations in Tear Protein Profile Reveal Pathogen-Induced Host Response to Fungal Infection