PXD003822

PXD003822 is an original dataset announced via ProteomeXchange.

Title	Human AML patient samples, Method Optimization, LC-MSMS
Description	We evaluated different in-solution and filter-aided sample preparation (FASP) proteomic workflows, and different enrichment strategies of phosphorylated peptides on acute myeloid leukemia (AML) patient samples. We also studied the effect of liquid nitrogen storage on the proteome and phosphoproteome of four AML patients.
HostingRepository	PRIDE
AnnounceDate	2016-04-26
AnnouncementXML	Submission_2016-04-26_07:18:42.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Elise Aasebo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; phosphorylated residue; 6x(13)C labeled L-arginine; 6x(13)C: 2x(15)N labeled L-lysine
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2016-03-21 02:26:26	ID requested
⏵ 1	2016-04-26 07:18:43	announced

Aaseb, ø E, Mjaavatten O, Vaudel M, Farag Y, Selheim F, Berven F, Bruserud Ø, Hernandez-Valladares M, Freezing effects on the acute myeloid leukemia cell proteome and phosphoproteome revealed using optimal quantitative workflows. J Proteomics, 145():214-225(2016) [pubmed]

curator keyword: Biomedical, Technical

submitter keyword: Human, AML cells, Sample Preparation, Label-free, Super-SILAC, phosphopeptide enrichment, LC-MSMS, LTQ-Orbitrap Elite

Frode Steingrimsen Berven
contact affiliation	Department of Biomedicine, PROBE, University of Bergen, Norway
contact email	frode.berven@uib.no
lab head
Elise Aasebo
contact affiliation	PROBE
contact email	elise.aasebo@uib.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/04/PXD003822

PRIDE project URI

• PRIDE
  1. PXD003822
     1. Label: PRIDE project
     2. Name: Human AML patient samples, Method Optimization, LC-MSMS