PXD003821

PXD003821 is an original dataset announced via ProteomeXchange.

Title	Arabidopsis Col-0 and cerk1 treated with chitosan or water
Description	Microbe-associated molecular pattern (MAMP)-triggered immunity (MTI) is the first layer of molecular defense encountered by pathogens when they attempt to infect plants. MTI is dependent on cell surface pattern-recognition receptors (PRR) which act upstream of mitogen-activated protein kinase (MAPK) pathways. Genetic screening and mutant knock-out lines have largely contributed to our knowledge of MTI. However, genetic screening is confined to phenotype-causing mutations and scarcely enables the discovery of redundantly-acting proteins. We sought to discover protein components that contribute in MTI, using a phenotype-independent approach to discern nucleus-localized proteins after MTI induction. We report on the nuclear proteome of Columbia-0 (Col-0) and chitin elicitor receptor kinase 1 (cerk1) mutant plants 15 min after MTI induction. Our approach revealed that MAMP-treated cerk1 plants had many proteins in common with Col-0-treated plants following chitosan treatment. cerk1 plants also manifested several unique proteins that were absent from Col-0 plants when elicited with chitosan indicating that they also perceive chitosan. Detailed analysis of the identified proteins revealed a nuclear accumulation of transcriptional regulators and transcription factors, DNA-modifying enzymes, RNA-binding proteins and ribosomal proteins. No novel MAPKs were found although Receptor for activated C kinase 1, a scaffold protein involved in defense, and a nucleotide binding leucine-rich repeat protein, implicated in resistance to Leptosphaeria maculans, were discovered in the nucleus of chitosan-treated plants and absent from water-treated control plants.
HostingRepository	PRIDE
AnnounceDate	2018-10-26
AnnouncementXML	Submission_2018-10-26_02:34:38.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003821
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hugo Germain
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	Ammonia-loss; Glu->pyro-Glu; Deamidated; Dehydrated; Oxidation; Acetyl; Carbamidomethyl; Gln->pyro-Glu
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2016-03-21 02:03:03	ID requested
⏵ 1	2018-10-26 02:34:39	announced

Fakih Z, Ahmed MB, Letanneur C, Germain H, An unbiased nuclear proteomics approach reveals novel nuclear protein components that participates in MAMP-triggered immunity. Plant Signal Behav, 11(6):e1183087(2016) [pubmed]

curator keyword: Biological

submitter keyword: arabidopsis, PAMP- triggered immunity, chitosan, cerk1

Hugo Germain
contact affiliation	Departement de Chimie, Biochimie et Physisque, Université du Québec à Trois-Rivières
contact email	hugo.germain@uqtr.ca
lab head
Hugo Germain
contact affiliation	Universite du Quebec a Trois-Rivieres
contact email	hugo.germain@uqtr.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003821
     1. Label: PRIDE project
     2. Name: Arabidopsis Col-0 and cerk1 treated with chitosan or water