PXD003804

PXD003804 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Next Generation Protein Sequencing
Description	NGPS is a method for de-novo, full-length protein sequencing in high throughput. The method is based on cleavage of the protein at semi-random sites by microwave-assisted acid hydrolysis (MAAH), enrichment of LC-MS/MS amenable peptides from the hydrolysate by solid-phase-extraction, LC-MS/MS analysis, de-novo long peptide tag sequencing of resulting peptides and assembly of peptide tags into consensus contigs.
HostingRepository	PRIDE
AnnounceDate	2017-04-03
AnnouncementXML	Submission_2017-04-03_02:28:54.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alon Savidor
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Equus caballus (Horse); NCBI TaxID: 9796;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2016-03-17 05:47:35	ID requested
⏵ 1	2017-04-03 02:28:55	announced

Publication List

Savidor A, Barzilay R, Elinger D, Yarden Y, Lindzen M, Gabashvili A, Adiv Tal O, Levin Y, Protein and Antibody Sequence Determination. Mol Cell Proteomics, 16(6):1151-1161(2017) [pubmed]

Savidor A, Barzilay R, Elinger D, Yarden Y, Lindzen M, Gabashvili A, Adiv Tal O, Levin Y, Protein and Antibody Sequence Determination. Mol Cell Proteomics, 16(6):1151-1161(2017) [pubmed]

Keyword List

curator keyword: Technical
submitter keyword: MAAH, microwave, de-novo, full length, NGPS

curator keyword: Technical

submitter keyword: MAAH, microwave, de-novo, full length, NGPS

Contact List

Yishai Levin
contact affiliation	The Nancy and Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science
contact email	yishai.levin@weizmann.ac.il
lab head
Alon Savidor
contact affiliation	The Weizmann Institute of Science
contact email	alon.savidor@weizmann.ac.il
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/04/PXD003804
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/04/PXD003804

PRIDE project URI

Repository Record List

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