PXD003802

PXD003802 is an original dataset announced via ProteomeXchange.

Title	S-nitrosation of proteins relevant to Alzheimer’s disease during early stages of neurodegeneration
Description	Protein S-nitrosation (SNO-protein) is a post-translational modification in which a cysteine (Cys) residue is modified by nitric oxide (SNO-Cys). SNO-proteins impact many biological systems, but their identification has been technically challenging. We developed a chemical proteomic strategy - SNOTRAP (SNO trapping by triaryl phosphine) -that allows improved identification of SNO-proteins by mass spectrometry. We found that S-nitrosation is elevated during early stages of neurodegeneration, preceding cognitive decline. We identified changes in the SNOproteome during early neurodegeneration that are potentially relevant for synapse function, metabolism, and Alzheimer’s disease pathology. SNO-proteome analysis further reveals a potential linear motif for SNO-Cys sites that are altered during neurodegeneration. Our strategy can be applied to multiple cellular and disease contexts and can reveal signaling networks that aid drug development.
HostingRepository	PRIDE
AnnounceDate	2016-03-31
AnnouncementXML	Submission_2016-04-04_06:27:33.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	John Wishnok
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	N-ethylmaleimide derivatized cysteine; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	6520 Quadrupole Time-of-Flight LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2016-03-17 05:28:25	ID requested
1	2016-03-31 09:17:59	announced
⏵ 2	2016-04-04 06:27:34	announced	Updated publication reference for PubMed record(s): 27035958.

Seneviratne U, Nott A, Bhat VB, Ravindra KC, Wishnok JS, Tsai LH, Tannenbaum SR, S-nitrosation of proteins relevant to Alzheimer's disease during early stages of neurodegeneration. Proc Natl Acad Sci U S A, 113(15):4152-7(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: S-nitrosylation, Alzheimer's disease, neurodegeneration

Steven R. Tannenbaum
contact affiliation	Department of Biological Engineering Department of Chemistry Massachusetts Institute of Technology Cambridge, MA 02139
contact email	srt@mit.edu
lab head
John Wishnok
contact affiliation	Massachusetts Institute of Technology
contact email	wishnok@mit.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/03/PXD003802

PRIDE project URI

• PRIDE
  1. PXD003802
     1. Label: PRIDE project
     2. Name: S-nitrosation of proteins relevant to Alzheimer’s disease during early stages of neurodegeneration