PXD003800

PXD003800 is an original dataset announced via ProteomeXchange.

Title	Epstein-Barr virus protein EB2 stimulates translation initiation of mRNAs through direct interactions with both PABP and eIF4G
Description	Epstein-Barr virus (EBV) expresses several mRNAs produced from intronless genes that could be unfavorably translated in front of cellular spliced mRNAs. To overpass these limitations, the virus encodes a RNA-binding protein (RBP), EB2, which is already known to facilitate nuclear mRNAs export and to increase their translational yield. Here we show that EB2 binds both nuclear and cytoplasmic cap-binding complexes (respectively, CBC and eIF4F), and the poly(A)-binding protein (PABP) to enhance translation initiation of a given mRNP. Interestingly, such effect can be obtained only if EB2 was initially bound to the native mRNPs in the nucleus. We also demonstrate that the EB2-eIF4F-PABP association renders the translation of these mRNPs less sensitive to inhibitors of initiation. Taken together, our new data suggest that EB2 binds and stabilizes cap-binding complexes to increase mRNP translation and demonstrate the importance of the mRNP assembly process in the nucleus to promote translation in the cytoplasm.
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_11:34:33.748.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003800
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Frederic DELOLME
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ XL ETD

Revision	Datetime	Status	ChangeLog Entry
0	2016-03-17 01:19:57	ID requested
⏵ 1	2024-10-08 11:34:34	announced

10.1128/JVI.01917-17;

10.6019/PXD003800;

curator keyword: Biomedical

submitter keyword: LC-MSMS, EB2-BMLF1,Epstein-Barr virus, translation

Frederic DELOLME
contact affiliation	UMS3444, Protein Science Facility, FRANCE
contact email	frederic.delolme@ibcp.fr
lab head
Frederic DELOLME
contact affiliation	CNRS UMS3444 Protein Science Facility
contact email	frederic.delolme@ibcp.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD003800

PRIDE project URI

• PRIDE
  1. PXD003800
     1. Label: PRIDE project
     2. Name: Epstein-Barr virus protein EB2 stimulates translation initiation of mRNAs through direct interactions with both PABP and eIF4G