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PXD003790

PXD003790 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHLA peptides (LC-MSMS) induced by Decitabine in Glioblastoma cell lines
DescriptionTreatment of cancer cells with anti-cancer drugs often fails to achieve complete remission. Yet, such drug treatments may induce alteration in the tumor’s gene expression patterns, including those of Cancer/Testis Antigens (CTA). The degradation products of such antigens can be presented as HLA peptides on the surface of the tumor cells and be developed into anti-cancer immunotherapeutics. For example, the DNA methyl transferase inhibitor, 5-aza-2'-deoxycytidine (Decitabine) has limited anti-tumor efficacy, yet it induces the expression of many genes, including CTAs that are normally silenced in the healthy adult tissues. In this study, the presentation of many new HLA peptides derived from CTAs and induced by Decitabine was demonstrated in three human Glioblastoma cell lines. Such presentation of CTA-derived HLA peptides can be exploited for development of new treatment modalities, combining drug treatment with anti-CTA targeted immunotherapy. The Decitabine-induced HLA peptidomes include many CTAs that are not normally detected in healthy tissues or in cancer cells, unless treated with the drug. In addition, the study included large-scale analyses of the simultaneous effects of Decitabine on the transcriptomes, proteomes and HLA peptidomes of the human Glioblastoma cells. It demonstrates the poor correlations between these three levels of gene expression, both in their total levels and in their response to the drug.
HostingRepositoryPRIDE
AnnounceDate2017-10-24
AnnouncementXMLSubmission_2017-10-24_01:12:50.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBracha Shraibman
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; acetylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-03-15 03:06:05ID requested
12016-07-18 00:54:51announced
22016-10-27 04:01:05announcedUpdated project metadata.
32016-11-09 02:27:33announcedUpdated project metadata.
42017-10-24 01:12:51announcedUpdated project metadata.
Publication List
Shraibman B, Kadosh DM, Barnea E, Admon A, Human Leukocyte Antigen (HLA) Peptides Derived from Tumor Antigens Induced by Inhibition of DNA Methylation for Development of Drug-facilitated Immunotherapy. Mol Cell Proteomics, 15(9):3058-70(2016) [pubmed]
Keyword List
ProteomeXchange project tag: Human Immuno-Peptidome Project (HUPO-HIPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project
curator keyword: Biomedical
submitter keyword: HLA peptides, Glioblastoma, Decitabine
Contact List
Arie Admon
contact affiliationDepartment of Biology, Technion - Israel Institute of Technology, Haifa, Israel
contact emailadmon@tx.technion.ac.il
lab head
Bracha Shraibman
contact affiliationDepartment of Biology, Technion- Israel Institute of Technology
contact emailbrachash85@gmail.com
dataset submitter
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Dataset FTP location
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PRIDE project URI
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