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PXD003788

PXD003788 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTemporal regulation of Lsp1 O-GlcNAcylation and phosphorylation duringactivated Bcellsapoptosis
DescriptionHere, we decipher the molecular mechanisms bridging B cell activation and apoptosis mediated by post-translational modification(PTM). We found that O-GlcNAcase inhibition enhances B cell activation and apoptosisinduced by B cell receptor (BCR)cross-linking. This proteome-scale analysis of the functional interplay between protein O-GlcNAcylation and phosphorylation in stimulated mouse primary B cells identified313O-GlcNAcylation-dependent phosphositeson 224 phosphoproteins.Among these phosphoproteins, temporal regulation ofthe O-GlcNAcylation and phosphorylation of lymphocyte-specific protein-1 (Lsp1) is a key switch that triggersapoptosis in activated B cells. O-GlcNAcylation at S209 of Lsp1, identified as the major O-GlcNAc site on Lsp1 by both electron-transfer dissociationand collision-induced dissociationtandem mass spectrometry,was a prerequisite for the recruitment of its kinase, PKC-β1, to induce S243 phosphorylation, leading to ERK activation and down-regulation of BCL-2 and BCL-xL. Thus, we demonstrate the critical PTM interplay of Lsp1 that transmits signals for initiating apoptosis after BCR ligation.
HostingRepositoryPRIDE
AnnounceDate2016-09-26
AnnouncementXMLSubmission_2016-09-26_04:38:51.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHsin-Yi Wu
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListphosphorylated residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-03-15 02:07:45ID requested
12016-09-26 04:38:52announced
Publication List
Wu JL, Wu HY, Tsai DY, Chiang MF, Chen YJ, Gao S, Lin CC, Lin CH, Khoo KH, Chen YJ, Lin KI, Temporal regulation of Lsp1 O-GlcNAcylation and phosphorylation during apoptosis of activated B cells. Nat Commun, 7():12526(2016) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: O-GlcNAcylation, phosphoproteome, B cell, apoptosis
Contact List
Yu-Ju Chen
contact affiliationInstitute of Chemistry, Academia Sinica
contact emailyujuchen@gate.sinica.edu.tw
lab head
Hsin-Yi Wu
contact affiliationAcademia Sinica
contact emailclairewu.wu@gmail.com
dataset submitter
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