PXD003786

PXD003786 is an original dataset announced via ProteomeXchange.

Title	Mineral-bound ostrich eggshell peptides survive for up to 3.8 Ma in equatorial Africa
Description	Claims for exceptional preservation of biomolecules in the fossil record are contested. Here we demonstrate the role of surface stabilisation in significantly prolonging protein sequence survival to ~3.8 million years. The intracrystalline environment of calcite ostrich (Struthionidae) eggshell encapsulates uterine proteins and molecular dynamics simulations of struthiocalcin-1 & -2, the dominant proteins within the eggshell, reveal that they bind to the mineral surface in distinct domains. By ~3.8 million years the struthiocalcin-1 domain with the lowest calculated binding energy is selectively preserved in eggshell samples from equatorial Africa. Sequence survival is explained by entropy loss of the peptide and water, lowering the effective temperature of the local environment at the peptide mineral interface.
HostingRepository	PRIDE
AnnounceDate	2018-07-16
AnnouncementXML	Submission_2018-07-16_07:01:56.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Beatrice Demarchi
SpeciesList	scientific name: Struthio camelus (Ostrich); NCBI TaxID: 8801;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-03-14 06:17:28	ID requested
⏵ 1	2018-07-16 07:01:56	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: eggshell, proteomics, LC-MS/MS, molecular dynamics, struthiocalcin, mineral binding

Matthew Collins
contact affiliation	BioArCh, Department of Archaeology, University of York
contact email	matthre.collins@york.ac.uk
lab head
Beatrice Demarchi
contact affiliation	University of york
contact email	beatrice.demarchi@york.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/07/PXD003786

PRIDE project URI

• PRIDE
  1. PXD003786
     1. Label: PRIDE project
     2. Name: Mineral-bound ostrich eggshell peptides survive for up to 3.8 Ma in equatorial Africa