PXD003785

PXD003785 is an original dataset announced via ProteomeXchange.

Title	Analysis of human sperm surface glycoproteins which can interact with galectin-1
Description	Herein, we constructed a human lectin microarray with 60 human lectin and lectin-like proteins. All of the lectins and lectin-like proteins were purified from yeast, and most showed binding to human glycans. To demonstrate the applicability of the human lectin microarray, human sperm were probed on the microarray and strong bindings were observed for several lectins, including galectin-1, 7, 8, GalNAc-T6, and ERGIC-53 (LMAN1). These bindings were validated by flow cytometry and fluorescence immunostaining. Further, mass spectrometry analysis showed that galectin-1 binds several membrane proteins including HSP90. Finally, functional assays showed that binding of galectin-8 could significantly enhance the acrosome reaction within human sperms. To our knowledge, this is the first construction of a human lectin microarray, and we anticipate it will find wide use for a range of human or mammalian studies, alone or in combination with plant lectin microarrays.
HostingRepository	PRIDE
AnnounceDate	2018-10-26
AnnouncementXML	Submission_2018-10-26_02:22:30.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003785
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yangyang Sun
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2016-03-14 05:22:56	ID requested
⏵ 1	2018-10-26 02:22:31	announced

Sun Y, Cheng L, Gu Y, Xin A, Wu B, Zhou S, Guo S, Liu Y, Diao H, Shi H, Wang G, Tao SC, A Human Lectin Microarray for Sperm Surface Glycosylation Analysis. Mol Cell Proteomics, 15(9):2839-51(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: Human sperm, Galectin-1, NLM

Sheng-ce Tao
contact affiliation	Shanghai Center for Systems Biomedicine Shanghai Jiaotong University B207, Bldg for Systems Biomedicine 800 Dongchuan Rd Shanghai, China, 200240
contact email	taosc@sjtu.edu.cn
lab head
Yangyang Sun
contact affiliation	Shanghai Jiao Tong University
contact email	sunyangyang06@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD003785

PRIDE project URI

• PRIDE
  1. PXD003785
     1. Label: PRIDE project
     2. Name: Analysis of human sperm surface glycoproteins which can interact with galectin-1