PXD003779

PXD003779 is an original dataset announced via ProteomeXchange.

Title	Differential protein expression of midgut proteins of An. culicifacies species A and species B using iTRAQ approach
Description	Background: Mosquito midgut is an important target for the host parasite interaction studies as this major immune organ plays a role in parasite growth, maturation and vector competence. Most important rural malaria vector of India is Proteomic approaches coupled with bioinformatics analysis have been used to study expression of functional proteins/enzymes of An. culicifacies susceptible and refractory species midgut in order to understand the mechanism of refractoriness that may help in contributing to unravel the host pathogen interactions Methodology/Principle findings: Basic shot gun proteomics approaches along with iTRAQ based differential studies followed by LC/MS/MS were employed to study the proteome of sugar fed An. culicifacies midgut. Further bioinformatics analysis was carried out using Gene ontology, SMART analysis, CELLO algorithm etc. Here proteins identified with iTRAQ Ratio more than 1.5 was considered up regulated and less than 0.67 were considered down regulated. Ratio from 1.5 – 0.67 were considered as moderate to no change. Using these criteria we observed total 80 proteins in An. culicifacies midgut and when compared susceptible species against refractory species, total 30 proteins were found to be downregulated. 3 proteins were found to be upregulated in refractory species Conclusion/Significance: Based on the present data a significant increase in number of proteins in midgut of refractory An. culicifacies species B were found that may conclude that these proteins may be responsible for the inhibiting parasite growth and linked to the melanization of oocysts or parasite lysis mechanisms in natural populations of refractory mosquito. Hence the progress of our studies at protein level suggests that these identified annotated putative proteins/enzymes may help to explore natural vector-parasite systems and reveal valuable insights into the mechanism of refractoriness which in turn further useful for bringing of novel strategies for control of malaria.
HostingRepository	PRIDE
AnnounceDate	2018-10-24
AnnouncementXML	Submission_2018-10-24_08:01:06.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003779
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	sonam vijay
SpeciesList	scientific name: Anopheles culicifacies B; NCBI TaxID: 63367; scientific name: Anopheles culicifacies A; NCBI TaxID: 63366;
ModificationList	Methylthio; Oxidation; iTRAQ8plex
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-03-14 03:45:02	ID requested
⏵ 1	2018-10-24 08:01:07	announced

Vijay S, Rawal R, Kadian K, Singh J, Adak T, Sharma A, Proteome-wide analysis of Anopheles culicifacies mosquito midgut: new insights into the mechanism of refractoriness. BMC Genomics, 19(1):337(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: Anopheles, iTRAQ method, LC/MS/MS, Midgut

Arun Sharma
contact affiliation	Scientist F Protein Biochemistry and Structural Biology head
contact email	arushar@gmail.com
lab head
sonam vijay
contact affiliation	National Institute of Malaria Research
contact email	sonam.vijay@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD003779

PRIDE project URI

• PRIDE
  1. PXD003779
     1. Label: PRIDE project
     2. Name: Differential protein expression of midgut proteins of An. culicifacies species A and species B using iTRAQ approach