PXD003774

PXD003774 is an original dataset announced via ProteomeXchange.

Title	In-cell isotope-coded labeling and LC-MS/MS analysis probe warfarin inhibition mechanism
Description	Warfarin targets human vitamin K epoxide reductase (hVKOR), a redox enzyme in the membrane of endoplasmic reticulum (ER), to prevent the formation of blood clots. Although warfarin has been a popular medication since 1954, its mechanism of action is still unclear. A fundamental issue is the controversial orientation of transmembrane helices (TM) in hVKOR. Stable isotope-coded reagents were usedto label VKOR in free, mutated, and warfarin-binding states directly in the cellular environment, followed by LC-MS/MS bottom-up approach to investigate the warfarin binding mechanism.
HostingRepository	PRIDE
AnnounceDate	2017-03-30
AnnouncementXML	Submission_2017-03-30_01:35:45.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003774
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Weidong Cui
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	quaternary amine labeling reagent heavy form (+3amu) (N-term & K); N-ethylmaleimide derivatized cysteine; hydrolyzed N-ethylmaleimide cysteine adduct; O4-arabinosyl-L-hydroxyproline; monohydroxylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-03-11 06:11:06	ID requested
⏵ 1	2017-03-30 01:35:49	announced

Shen G, Cui W, Zhang H, Zhou F, Huang W, Liu Q, Yang Y, Li S, Bowman GR, Sadler JE, Gross ML, Li W, Warfarin traps human vitamin K epoxide reductase in an intermediate state during electron transfer. Nat Struct Mol Biol, 24(1):69-76(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: warfarin, human vitamin K epoxide reuctase, N-ethylmeleimide, isotope, bottom-up proteomics

Michael L. Gross
contact affiliation	Washington University in Saint Louis
contact email	mgross@wustl.edu
lab head
Weidong Cui
contact affiliation	Washington University in Saint Louis
contact email	cui@wustl.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/03/PXD003774

PRIDE project URI

• PRIDE
  1. PXD003774
     1. Label: PRIDE project
     2. Name: In-cell isotope-coded labeling and LC-MS/MS analysis probe warfarin inhibition mechanism