PXD003762 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Probing the HIV-Host Interactome in Infected Cells |
Description | Although genetically compact, HIV-1 commandeers vast arrays of cellular machinery that sustain and protect it during cycles of viral outgrowth. Transposon-mediated saturation linker scanning mutagenesis was used to isolate fully replication-competent viruses harboring a potent foreign epitope tag. Using these viral isolates, we performed differential isotopic labeling and affinity-capture mass spectrometric analyses on samples obtained from cultures of human lymphocytes to classify the vicinal interactomes of the viral Env and Vif proteins as they occur during natural infection. Importantly, interacting proteins were recovered without bias, regardless of their potential for positive, negative or neutral impact on viral replication. Specific host associations made with trimerized Env during its biosynthesis, at virological synapses, with innate immune effectors and with certain cellular signaling pathways (ex. Notch1) were identified. We also defined Vif associations with host proteins involved in the control of nuclear transcription and nucleoside biosynthesis as well as those that interact both stably and transiently with the cytoplasmic protein degradation apparatus. Our approach is broadly applicable to elucidating pathogen-host interactomes, providing high certainty identification of interactors by their direct access during cycling infection. Understanding the pathophysiological consequences of these associations is likely to provide strategic targets for antiviral intervention. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:05:49.445.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Todd Greco |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-03-10 01:13:24 | ID requested | |
1 | 2016-07-06 02:15:38 | announced | |
⏵ 2 | 2024-10-22 04:05:54 | announced | 2024-10-22: Updated project metadata. |
Publication List
Luo Y, Jacobs EY, Greco TM, Mohammed KD, Tong T, Keegan S, Binley JM, Cristea IM, Feny, รถ D, Rout MP, Chait BT, Muesing MA, HIV-host interactome revealed directly from infected cells. Nat Microbiol, 1(7):16068(2016) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: protein interactions, AP-MS, Env, I-DIRT, Vif |
Contact List
Ileana Cristea |
contact affiliation | Dept. of Molecular Biology, Princeton, NJ USA 08544 |
contact email | icristea@princton.edu |
lab head | |
Todd Greco |
contact affiliation | Molecular Biology, Princeton University |
contact email | tgreco@princeton.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003762
- Label: PRIDE project
- Name: Probing the HIV-Host Interactome in Infected Cells