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PXD003762

PXD003762 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProbing the HIV-Host Interactome in Infected Cells
DescriptionAlthough genetically compact, HIV-1 commandeers vast arrays of cellular machinery that sustain and protect it during cycles of viral outgrowth. Transposon-mediated saturation linker scanning mutagenesis was used to isolate fully replication-competent viruses harboring a potent foreign epitope tag. Using these viral isolates, we performed differential isotopic labeling and affinity-capture mass spectrometric analyses on samples obtained from cultures of human lymphocytes to classify the vicinal interactomes of the viral Env and Vif proteins as they occur during natural infection. Importantly, interacting proteins were recovered without bias, regardless of their potential for positive, negative or neutral impact on viral replication. Specific host associations made with trimerized Env during its biosynthesis, at virological synapses, with innate immune effectors and with certain cellular signaling pathways (ex. Notch1) were identified. We also defined Vif associations with host proteins involved in the control of nuclear transcription and nucleoside biosynthesis as well as those that interact both stably and transiently with the cytoplasmic protein degradation apparatus. Our approach is broadly applicable to elucidating pathogen-host interactomes, providing high certainty identification of interactors by their direct access during cycling infection. Understanding the pathophysiological consequences of these associations is likely to provide strategic targets for antiviral intervention.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:05:49.445.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTodd Greco
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-03-10 01:13:24ID requested
12016-07-06 02:15:38announced
22024-10-22 04:05:54announced2024-10-22: Updated project metadata.
Publication List
Luo Y, Jacobs EY, Greco TM, Mohammed KD, Tong T, Keegan S, Binley JM, Cristea IM, Feny, รถ D, Rout MP, Chait BT, Muesing MA, HIV-host interactome revealed directly from infected cells. Nat Microbiol, 1(7):16068(2016) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: protein interactions, AP-MS, Env, I-DIRT, Vif
Contact List
Ileana Cristea
contact affiliationDept. of Molecular Biology, Princeton, NJ USA 08544
contact emailicristea@princton.edu
lab head
Todd Greco
contact affiliationMolecular Biology, Princeton University
contact emailtgreco@princeton.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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