PXD003754

PXD003754 is an original dataset announced via ProteomeXchange.

Title	Global Membrane Protein Interactome Analysis using In vivo Crosslinking and MS-based Protein Correlation Profiling
Description	We present a methodology using in vivo crosslinking combined with HPLC-MS for the global analysis of endogenous protein complexes by protein correlation profiling. Formaldehyde crosslinked protein complexes were extracted with high yield using denaturing buffers that maintained complex solubility during chromatographic separation. We show this efficiently detects both integral membrane and membrane-associated protein complexes, in addition to soluble complexes, allowing identification and analysis of complexes not accessible in native extracts. We compare the protein complexes detected by HPLC-MS protein correlation profiling in both native and formaldehyde crosslinked U2OS cell extracts. These proteome-wide datasets of both in vivo crosslinked and native protein complexes from U2OS cells are freely available via a searchable online database (www.peptracker.com/epd).
HostingRepository	PRIDE
AnnounceDate	2016-04-27
AnnouncementXML	Submission_2016-04-27_13:05:37.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vackar Afzal
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	deamidated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-03-08 02:34:41	ID requested
⏵ 1	2016-04-27 13:05:38	announced

Larance M, Kirkwood KJ, Tinti M, Brenes Murillo A, Ferguson MA, Lamond AI, Global Membrane Protein Interactome Analysis using In vivo Crosslinking and Mass Spectrometry-based Protein Correlation Profiling. Mol Cell Proteomics, 15(7):2476-90(2016) [pubmed]

curator keyword: Biological, Technical

submitter keyword: membrane protein

interactome analysis, proteomics

protein complexes

protein crosslinking

mass spectrometry

Angus Lamond
contact affiliation	University of Dundee
contact email	a.i.lamond@dundee.ac.uk
lab head
Vackar Afzal
contact affiliation	School of Life Sciences
contact email	v.z.afzal@dundee.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003754
     1. Label: PRIDE project
     2. Name: Global Membrane Protein Interactome Analysis using In vivo Crosslinking and MS-based Protein Correlation Profiling