PXD003753

PXD003753 is an original dataset announced via ProteomeXchange.

Title	Botrytis cinerea phosphoproteome.
Description	The phytopathogen Botrytis cinerea is a ubiquitous fungus with a high capacity to adapt its metabolism to different hosts and environmental conditions in order to deploy a variety of virulence and pathogenicity factors and develop a successful plant infection. Here we report the first comparative phosphoproteomic study of B. cinerea, aimed to analyze the phosphoprotein composition of the fungus and its changes under different phenotypical conditions induced by two different carbon sources as plant based elicitors: glucose and deproteinized tomato cell wall (TCW). A total of 2854 and 2269 different phosphosites (2883 and 1137 phosphopeptides) were identified in glucose and TCW respectively, which map to 1338 phosphoproteins in glucose and 733 in TCW. Out of the identified phosphoproteins, 173 were exclusively found when glucose was the only carbon source and 11 when the carbon source was TCW. Differences in the pattern of phosphorylation-sites were also detected according to the carbon source. Gene ontology classification of the identified phosphoproteins showed that most of the characteristic proteins of the different carbon sources were related to signaling and transmembrane transport, thus highlighting the importance of these processes in the fungal adaptation to the surrounding conditions.
HostingRepository	PRIDE
AnnounceDate	2016-04-14
AnnouncementXML	Submission_2016-04-14_02:34:39.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cristina Chiva
SpeciesList	scientific name: Botryotinia fuckeliana (Noble rot fungus) (Botrytis cinerea); NCBI TaxID: 40559;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-03-08 02:06:55	ID requested
⏵ 1	2016-04-14 02:34:40	announced

Li, ñ, eiro E, Chiva C, Cantoral JM, Sabido E, Fern, á, ndez-Acero FJ, Phosphoproteome analysis of B. cinerea in response to different plant-based elicitors. J Proteomics, 139():84-94(2016) [pubmed]

curator keyword: Biological

submitter keyword: Botrytis cinerea, phosphoproteome, plant pathogenicity

Francisco Javier Fernández Acero
contact affiliation	Laboratorio de Microbiología Centro Andaluz de Investigaciones Vitivinícolas (CAIV) Facultad de Ciencias del Mar y Ambientales (CASEM) Universidad de Cádiz
contact email	franciscojavier.fernandez@uca.es
lab head
Cristina Chiva
contact affiliation	Center of genomic regulation
contact email	chiva.cristina@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/04/PXD003753

PRIDE project URI

• PRIDE
  1. PXD003753
     1. Label: PRIDE project
     2. Name: Botrytis cinerea phosphoproteome.