PXD003751

PXD003751 is an original dataset announced via ProteomeXchange.

Title	Central role of phosphorylation in the circadian control of liver metabolism
Description	Circadian clocks are endogenous oscillators that drive organismal metabolism and physiology. Here, we report the first global in vivo quantification of circadian phosphorylation rhythms in mammals. Of more than 20,000 in vivo phosphosites, 25% significantly oscillate in the mouse liver, including novel sites on core clock proteins. Analyzing kinase substrate motifs, we find that the EGF/RAS/ERK pathway is predominantly activated during the day and the AKT/mTOR/p70S6K at night. The extent and amplitude of phosphorylation cycles dominate the rhythms of transcript and protein abundance. A dominant regulatory role for phosphorylation-dependent circadian tuning of signaling pathways allows the organism to rapidly and economically respond to daily changes in nutrient availability and integrate different signaling triggers.
HostingRepository	PRIDE
AnnounceDate	2016-10-18
AnnouncementXML	Submission_2019-02-12_08:05:26.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mario Oroshi
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-03-07 08:11:54	ID requested
1	2016-10-18 04:32:24	announced
⏵ 2	2019-02-12 08:05:28	announced	Updated publication reference for PubMed record(s): 27818261.

Robles MS, Humphrey SJ, Mann M, Phosphorylation Is a Central Mechanism for Circadian Control of Metabolism and Physiology. Cell Metab, 25(1):118-127(2017) [pubmed]

curator keyword: Biological

submitter keyword: circadian, phosphoproteomics, liver metabolism

matthias mann
contact affiliation	Dept. Proteomics and Signal Transduction Max-Planck Institute of Biochemistry Am Klopferspitz 18 D-82152 Martinsried (near Munich)
contact email	mmann@biochem.mpg.de
lab head
Mario Oroshi
contact affiliation	Proteomics
contact email	oroshi@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD003751

PRIDE project URI

• PRIDE
  1. PXD003751
     1. Label: PRIDE project
     2. Name: Central role of phosphorylation in the circadian control of liver metabolism