PXD003748

PXD003748 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	RNA polymerase III interactome
Description	Many regulatory proteins and complexes have been identified which influence transcription by RNA polymerase (pol) II with a fine precision. In comparison, only a few regulatory proteins are known for pol III, which transcribes mostly house-keeping and non-coding RNAs. Yet, pol III transcription is precisely regulated under various stress conditions like starvation. We used proteomic approaches and pol III transcription complex components TFIIIC (Tfc6), pol III (Rpc128) and TFIIIB (Brf1) as baits to find identify the potential interactors through mass spectrometry-based proteomics. A large number of proteins were found in the interactome, which includes known chromatin modifiers, factors and regulators of transcription by pol I and pol II.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:05:51.271.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003748
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yohann Couté
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2016-03-07 02:49:44	ID requested
1	2019-01-02 02:35:03	announced
⏵ 2	2024-10-22 04:05:59	announced	2024-10-22: Updated project metadata.

Publication List

10.1016/j.gene.2018.12.037;
Bhalla P, Vernekar DV, Gilquin B, Cout, é Y, Bhargava P, Interactome of the yeast RNA polymerase III transcription machinery constitutes several chromatin modifiers and regulators of the genes transcribed by RNA polymerase II. Gene, 702():205-214(2019) [pubmed]
10.6019/PXD003748;

10.1016/j.gene.2018.12.037;

Bhalla P, Vernekar DV, Gilquin B, Cout, é Y, Bhargava P, Interactome of the yeast RNA polymerase III transcription machinery constitutes several chromatin modifiers and regulators of the genes transcribed by RNA polymerase II. Gene, 702():205-214(2019) [pubmed]

10.6019/PXD003748;

Keyword List

curator keyword: Biological
submitter keyword: co-IPs, RNA polymerase III, nanoLC-MS/MS,S. cerevisiae

curator keyword: Biological

submitter keyword: co-IPs, RNA polymerase III, nanoLC-MS/MS,S. cerevisiae

Contact List

Virginie Brun
contact affiliation	EDyP
contact email	virginie.brun@cea.fr
lab head
Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD003748
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD003748

PRIDE project URI

Repository Record List

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