PXD003743

PXD003743 is an original dataset announced via ProteomeXchange.

Title	Proteome of silenced minichromosome maintenance protein 2 with siRNA on non-small cell lung cancer H1299 cells
Description	Lung cancer is the leading cause of cancer-related mortality. The two main lung cancer types are small cell lung cancer (SCLC) and non-SCLC (NSCLC), where NSCLC comprises about 80-85% of all lung cancer. Despite the introduction of improved treatments, the overall survival rate of lung cancer patients remains low. Further elucidation of the regulatory network perturbations between cancer-related genes and proteins is one promising route to alter this mortality trend. The deregulation of the DNA replication, cell cycle, proliferation and migration are the common factors that are involved in cancer development and progression, and therefore logical targets for analysis. Minichromosome maintenance 2(MCM2) is a DNA replication licensing factor, which belongs to the heterohexameric MCM2-7 complex. MCM2 has been proposed as an excellent proliferation marker in many types of cancer. Our study will establish a global functional distribution of identified proteins in silenced-MCM2 in H1299 NSCLC by the means of iTRAQ. Understanding the molecular basis of MCM2 in lung cancer cells enables us to discover alternative target for lung cancer therapy.
HostingRepository	PRIDE
AnnounceDate	2018-10-26
AnnouncementXML	Submission_2018-10-26_02:17:33.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	hoi yin cheung
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-03-06 13:41:44	ID requested
⏵ 1	2018-10-26 02:17:34	announced

Cheung CHY, Hsu CL, Chen KP, Chong ST, Wu CH, Huang HC, Juan HF, MCM2-regulated functional networks in lung cancer by multi-dimensional proteomic approach. Sci Rep, 7(1):13302(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: non small cell Lung cancer H1299 cells, minichromosome maintenance protein 2, RNA interference, Proteome, iTRAQ, LC-MS/MS

Hsueh-Fen Juan
contact affiliation	Institute of Molecular & Cellular Biology, National Taiwan University, Taiwan
contact email	yukijuan@ntu.edu.tw
lab head
hoi yin cheung
contact affiliation	national taiwan university
contact email	hycchantal@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD003743

PRIDE project URI

• PRIDE
  1. PXD003743
     1. Label: PRIDE project
     2. Name: Proteome of silenced minichromosome maintenance protein 2 with siRNA on non-small cell lung cancer H1299 cells